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Jonathan F. Fay, PhD

Academic Title:

Assistant Professor

Primary Appointment:

Biochemistry and Molecular Biology


108 Greene St Baltimore, MD 21201

Phone (Primary):


Education and Training

1995–1999      B.A., Biochemistry/Molecular Biology, Reed College

2006–2012      Ph.D., Biochemistry/Molecular Biology, Oregon Health & Science University

Research/Clinical Keywords

Membrane protein, structure, structural biology, cryo-EM, GPCR

Highlighted Publications


Fay, J. F., & Farrens, D. L. (2015). Structural dynamics and energetics underlying allosteric inactivation of the cannabinoid receptor CB1. Proceedings of the National Academy of Sciences, 112, 8469–8474.

Fay, J. F., Aleksandrov, L. A., Jensen, T. J., Cui, L. L., Kousouros, J. N., He, L., … Chen, J. Z. (2018). Cryo-EM Visualization of an Active High Open Probability CFTR Anion Channel. Biochemistry, 57, 6234–6246.

Cao, C., Kang H., Singh, I, Chen, H., Zhang, C, Ye, W., Liu, J., Gumpper, R., Bender, B., Slocum, S. Krumm, B., Lansu, K., , McCorvy, D., Kroeze, W., DiBerto, J Olsen, R., Huang, X., Zhang, S., Liu, Y. Kim, K., Jan, Y., Jin, J., Shoichet, B., Fay, J.F. and Roth., B., (2021) Structure, Function and Pharmacology of Human Itch GPCRs. Nature, 600, 170-175

Peck, J.V., J.F. Fay, and J.D. (2022)  Strauss, High-speed high-resolution data collection on a 200 keV cryo-TEM. IUCrJ, 9,2



Additional Publication Citations


Zhang, S., Chen, H., Zhang, C., Yang, Y., Popov, P., Liu, J., . . . Fay, J.F. Roth, B. L. Inactive and active state structures template selective tools for the human 5-HT5A receptor. Nat Struct Mol Biol (2022). doi:10.1038/s41594-022-00796-6

Gumpper RH, Fay JF, Roth BL. Molecular insights into the regulation of constitutive activity by RNA editing of 5HT2C serotonin receptors. Cell Rep. 2022 Aug 16;40(7):111211

Cao C, Barros-Álvarez X, Zhang S, Kim K, Dämgen MA, Panova O, Suomivuori CM, Fay JF, Zhong X, Krumm BE, Gumpper RH, Seven AB, Robertson MJ, Krogan NJ, Hüttenhain R, Nichols DE, Dror RO, Skiniotis G, Roth BL. Signaling snapshots of a serotonin receptor activated by the prototypical psychedelic LSD. Neuron. 2022 Oct 5;110(19):3154-3167


Research Interests

My lab’s attention is heavily focused on cryo-EM as a tool for structural biology and primarily centered around three topics of research. These include (1) structural studies of G protein-coupled receptors, (2) methods to improve and expedite cryo-EM structural studies, and (3) protein structure and function of ABC proteins.