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A specific role of integrin Mac-1 in accelerated macrophage efflux to the lymphatics 

Chunzhang Cao, Daniel A. Lawrence, Dudley K. Strickland, and Li Zhang

Department of Physiology, University of Maryland School of Medicine; and the Department of Surgery, University of Maryland School of Medicine

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In response to injury, monocytes migrate to the site of inflammation, where they differentiate into macrophages and participate in various biologic processes. However, their fate during the resolution of acute inflammation is not fully understood. Here, we show that inflammatory macrophages do not die locally by apoptosis; rather, they migrate across the peritoneal mesothelium to the lymphatics, through which they further migrate to the lymph nodes and to the blood circulation. Macrophage efflux is enhanced considerably on cell activation, and such accelerated macrophage migration is dependent specifically on integrin Mac-1, and can be blocked by addition of its antagonist. Thus, genetic inactivation of Mac-1 in mice inhibits the accelerated macrophage efflux from the inflammatory site to the lymphatics, but it does not compromise the accumulation of blood monocytes into the inflammatory site. Together, our study demonstrates that Mac-1 is involved specifically in the efflux of activated macrophages to the lymphatics, suggesting that Mac-1 may play an important role in the removal of local inflammatory macrophages and in their subsequent migration to the lymph nodes, a process that is critical to the development of the adaptive immunity.

Macrophage migration from the peritoneum to the lymph nodes. A mixture of PKH67-labeled WT and PKH26-labeled Mac-1-/- macrophages (i) or PKH67-labeled WT and PKH26-labeled WT macrophages (ii) were separately injected intraperitoneally into the WT mice, followed by intraperitoneal injections of PBS or LPS. Four hours later, the number of the adoptively transferred WT and Mac-1-/- macrophages within the peritoneum was analyzed by dual-color FACS analysis (A), and their migration into the lymph nodes was visualized by fluorescence microscopy of the corresponding frozen sections (B). The data shown are representative of 2 independent experiments.