20 Penn Street
Education and Training
B.Pharm Madras Medical College; Chennai, India
M.S. Department of Chemical Engineering (specialization in Biotechnology) from IIT Madras, Chennai, India
Dr. rer. nat. Neurobiochemistry, from Otto-Von- Guericke University, Magdeburg, Germany
The Lung epithelium is exposed to a large range of temperatures that vary siginificantly from the core body temperature. It is also exposed to a whole host of microbial agents and pollunants from the environment. This results in the activation of the heat shock response, a highly conserved cellular response to stress. This results in the activation of heat shock factor -1 (HSF1) that leads to the generation of heat shock proteins and modulation of the immune reponse of the organism.
We have been able to show that at febrile range hyperthermia (FRH) auguments the production of inflammatory cytokines in the presence of infections and results in the release of heat shock protes that function as potential agonist for Toll like receptors (TLRs).Under the above circumcustances there is an activation of the p38MAPK pathway too. This pathaway is involved in regulation of a number of cellular responses from cell motility in neutrophils, cytoskeletal changes in epithilal cells to maintainance of endothelial tight junctions.
Currrently we are involved in undestanding how hypo and hyperthermia modulate the activation of p38 and the downstream subtrates that are activated. This will help us better understand possible drugable targets in the treatment of conditions like sepsis and ARDS.
G protein coupled receptors, Acute Lung Injury, HSF1, Heat shock proteins, Epithelial Mesenchymal transitions, Mitochondria, Lung vascular permeability
Tulapurkar M.E, Laubinger W, Nahum V, Fischer B, Reiser G. Subtype specific internalization of P2Y1 and P2Y2 receptors induced by novel adenosine 5'-O-(1-boranotriphosphate) derivatives. Br J Pharmacol. 2004; 142(5): 869-78.
Tulapurkar M.E, Schafer R, Hanck T, Flores RV, Weisman GA, Gonzalez FA, Reiser G. Endocytosis mechanism of P2Y2 nucleotide receptor tagged with green fluorescent protein: clathrin and actin cytoskeleton-dependence. Cell Mol Life Sci. 2005; 62(12): 1388-99
Tulapurkar, M.E., Almutairy, E.A., Shah, N.G., He, J., Shapiro, P., Singh, I.S., Hasday, J.D. Febrile-Range Hyperthermia Augments Neutrophil Recruitment to Lung via Modulation of Endothelial and Neutrophil Function. Am J Respir Cell Mol Biol. 2012, 46(6): 807-14
List of Publications
- Tulapurkar M.E, Laubinger W, Nahum V, Fischer B, Reiser G. Subtype specific internalization of P2Y1 and P2Y2 receptors induced by novel adenosine 5'-O-(1-boranotriphosphate) derivatives. Br J Pharmacol. 2004; 142(5): 869-78.
- Tulapurkar M.E, Schafer R, Hanck T, Flores RV, Weisman GA, Gonzalez FA, Reiser G. Endocytosis mechanism of P2Y2 nucleotide receptor tagged with green fluorescent protein: clathrin and actin cytoskeleton-dependence. Cell Mol Life Sci. 2005; 62(12): 1388-99.
- Tulapurkar, M.E.; Zündorf, G. and Reiser, G. Internalisation and desensitization of a green fluorescence protein-tagged P2Y1 nucleotide receptor are differently controlled by inhibition of calmodulin-dependent protein kinase II. J Neurochem. 2006; 96(3): 624-3.
- Nahum V, Tulapurkar M, Levesque SA, Sevigny J, Reiser G, Fischer B. Diadenosine and diuridine poly(borano)phosphate analogues: synthesis, chemical and enzymatic stability, and activity at P2Y1 and P2Y2 receptors. (equal contribution) J Med Chem. 2006; 49(6): 1980-90 .
- Laubinger W, Tulapurkar M.E, Schafer R, Reiser G. Distinct mono- and dinucleotide-specific P2Y receptors in A549 lung epithelial cells: different control of arachidonic acid release and nitric oxide synthase expression. Eur J Pharmacol. 2006; 543(1-3): 1-7.
- Ecke D, Tulapurkar M.E, Nahum V, Fischer B, Reiser G. Opposite diastereoselective activation of P2Y(1) and P2Y(11) nucleotide receptors by adenosine 5'-O-(alpha-boranotriphosphate) analogues (equal contribution) . Br J Pharmacol. 2006; 149(4): 416-23.
- Kahlert S, Blasera T, Tulapurkar M, and Reiser G. P2Y receptor-activating nucleotides modulate cellular ROS production in dissociated hippocampal astrocytes and neurons in culture, independent of parallel cytosolic Ca2+ rise and change in mitochondrial potential. J Neurosci Res. 2007; 85(15): 3443-56.
- Nestor MW, Mok LP, Tulapurkar ME, Thompson SM. Plasticity of neuron-glial interactions mediated by astrocytic EphARs J Neurosci. 2007; 27(47): 12817-28.
- Ecke D, Hanck T, Tulapurkar ME, Schäfer R, Kassack M, Stricker R, Reiser G. Hetero-oligomerization of the P2Y11 receptor with the P2Y1 receptor controls the internalization and ligand selectivity of the P2Y11 receptor Biochem J. 2008; 409(1):107-16.
- Tulapurkar ME, Asiegbu BE, Singh IS, Hasday JD Hyperthermia in the febrile range induces HSP72 expression proportional to exposure temperature but not to HSF-1 DNA-binding activity in human lung epithelial A549 cells. Cell Stress Chaperones, 2009; 14:499-508.
- Shah, N.G., Tulapurkar, M.E., Singh, I.S., Shelhamer, J., Cowan, M.J. and Hasday, J.D. Prostaglandin E2 Potentiates Heat Shock-Induced Heat Shock Protein 72 Expression in A549 Cells. Prostaglandins and Other Lipid Mediators (equal contribution). Prostaglandins Other Lipid Mediat. 2010; 93(1-2): 1-7
- Hasday JD, Shah N, Mackowiak PA, Tulapurkar M, Nagarsekar A, Singh I. Fever, hyperthermia, and the lung: it's all about context and timing. Trans Am Clin Climatol Assoc. 2011; 122: 34-47. (Review)
- Sareh H, Tulapurkar ME, Shah NG, Singh IS, Hasday JD. Response of mice to continuous 5-day passive hyperthermia resembles human heat acclimation. Cell Stress Chaperones. 2011; 16(3): 297-307.
- Maity TK, Henry MM, Tulapurkar ME, Shah NG, Hasday JD, Singh IS. Distinct, gene-specific effect of heat shock on heat shock factor-1 recruitment and gene expression of CXC chemokine genes. Cytokine. 2011; 54(1):61-7.
- Tulapurkar, M.E., Singh, I.S., Hasday, J.D. Prolonged Exposure to Febrile-Range Hyperthermia Augments Neutrophil Recruitment to Lung During the Post-Exposure period. Int J Hyperthermia. 2011; 27(7):717-25.
- Tulapurkar, M.E., Almutairy, E.A., Shah, N.G., He, J., Shapiro, P., Singh, I.S., Hasday, J.D. Febrile-Range Hyperthermia Augments Neutrophil Recruitment to Lung via Modulation of Endothelial and Neutrophil Function. Am J Respir Cell Mol Biol. 2012, 46(6): 807-14.
- Shah, N.G., Tulapurkar, M.E., Singh, I.S.; Goldblum, S.E.; Shapiro, P.; Hasday, J.D. Febrile-range Hyperthermia Augments Reversible TNFα-induced Hyperpermeability in Human Microvascular Lung Endothelial Cells Int J Hyperthermia. 2012; 28(7):627-35.
- Nagarsekar, A.; Tulapurkar, M.E.; Singh, I.S.; Atamas, S.; Shah, N.G.; Hasday, J.D. Hyperthermia Promotes and Prevents Apoptosis through Distinct Mechanisms. Am J Respir Cell Mol Biol. 2012; 47(6): 824-33
- Bridges TM, Tulapurkar ME, Shah NG, Singh IS, Hasday JD. Tolerance for chronic heat exposure is greater in female than male mice. Int J Hyperthermia. 2012; 28 (8):747-55.
- Gupta, A., Cooper, Z., Tulapurkar, M.E Maity, T., Hasday, J.D., Singh, I.S. Toll-like Receptor Agonists Augment Febrile Range Hyperthermia-induced Heat Shock Protein 72 Expression and Extracellular Release J Biol Chem. 2013; 288(4):2756-66)
- Ramachandran, G., Tulapurkar, M.E, Harris KM, Arad G, Shirvan A, Shemesh R, Detolla LJ, Benazzi C, Opal SM, Kaempfer R, Cross AS. A peptide antagonist of CD28 signaling attenuates toxic shock and necrotizing soft tissue infection induced by Streptococcus pyogenes J Infect Dis. 2013; 207(12):1869-77
- Simon, R., Wang, J.Y., Boyd, M.K., Tulapurkar, M.E, Ramachandran, G., Tennant, S.M., Pasetti, M., Galen, J.E., Levine, M.M. Sustained protection in mice with low doses of Salmonella Enteritidis core and O polysaccharide-flagellin glycoconjugates. PLoS One. 2013;8(5):e64680.
- Roche, J.A, Tulapurkar, M.E., Mueller, A.L., van Rooijen, N., Hasday, J.D., Lovering, R. M., Bloch, R.J. Myofiber Damage Precedes Macrophage Infiltration after Contraction-Induced Injury in Dysferlin-null Muscle. (equal authorship). Am J Pathol. 2015;185 (6):1686-98.
- Ramachandran, G., Darren Perkins, D, Schmidlein, P.J., Tulapurkar, M.E, Tennant, S. Invasive Salmonella Typhimurium ST313 with naturally attenuated flagellin elicits reduced inflammation and replicates within macrophages. PLoS Negl Trop Dis. 2015; 9(1):e3394.
- Tulapurkar ME, Ramarathnam A, Hasday JD, Singh IS Bacterial Lipopolysaccharide Augments Febrile-Range Hyperthermia-Induce Heat Shock Protein 70 Expression And Extracellular Release In Human THP1 Cells. PLoS One. 2015;10 (2):e0118010
- Ramachandran, G., Tennant, S.M., Boyd, M.A.; Wang, J.Y.; Tulapurkar, M.E.; Pasetti, M.F.; Levine, M.M.; Simon, R. Functional activity of antibodies directed towards flagellin proteins of non-typhoidal Salmonella. PLoS One. 2016 Mar 21;11(3):e0151875
- Scheraga RG, Thompson C, Tulapurkar ME, Nagarsekar AC, Cowan M, Potla R, Sun J, Cai R, Logun C, Shelhamer J, Todd NW, Singh IS, Luzina IG, Atamas SP, Hasday JD. Activation of Heat Shock Response Augments Fibroblast Growth Factor-1 Expression in Wounded Lung Epithelium: A Possible Mechanism for Progressive Lung Fibrosis. Am J Physiol Lung Cell Mol Physiol. 2016 Nov 1;311(5):L941-L955
- Sharma S, Mishra R, Bigham GE, Wehman B, Khan MM, Xu H, Saha P, Goo YA, Datla SR, Chen L, Tulapurkar ME, Taylor BS, Yang P, Karathanasis S, Goodlett DR, Kaushal S A Deep Proteome Analysis Identifies the Complete Secretome as the Functional Unit of Human Cardiac Progenitor Cells. Circ Res. 2017 Mar 3;120(5):816-834.
- Shah NG, Tulapurkar ME, Ramarathnam A, Brophy A, Martinez R 3rd, Hom K, Hodges T, Samadani R, Singh IS, MacKerell AD Jr, Shapiro P, Hasday JD. Novel Noncatalytic Substrate-Selective p38α-Specific MAPK Inhibitors with Endothelial-Stabilizing and Anti-Inflammatory Activity. J Immunol. 2017 Apr 15;198(8):3296-3306
- Potla R, Tulapurkar ME, Luzina IG, Atamas SP, Singh IS, Hasday JD Exposure to febrile-range hyperthermia potentiates Wnt signalling and epithelial-mesenchymal transition gene expression in lung epithelium.. Int J Hyperthermia. 2017 Apr 26:1-10.
- Ke Y, Oskolkova OV, Sarich N, Tian Y, Sitikov A, Tulapurkar ME, Son S, Birukova AA, Birukov KG Effects of prostaglandin lipid mediators on agonist-induced lung endothelial permeability and inflammation. Am J Physiol Lung Cell Mol Physiol. 2017 Oct 1;313(4):L710-L721.