20 Penn street, Baltimore,MD 21201
Education and Training
1977~1982 M.D. China Medical University
1983~1986 Internship & Residency Department of Neurology, First Hospital of China Medical
1986~1988: Fellowship, Department of Neurology, China Medical University.
1991~1994: Fellowship, Neurovirology Lab, Department of Neurology, School of Medicine, Johns
I have the expertise includes medical research, training and motivation necessary to successfully carry out the proposed research project. I have a broad background in molecular biology, immunology, cell biology, and data analysis.I have considerable experience and specific expertise in several research areas including the regulation of bladder epithelial cell growth in normal and inflammatory conditions. My research focused on isolation and characterization of APF, a novel peptide associated with interstitial cystitis, and its potential role in bladder epithelial cell proliferation and migration . I have made a number of important contributions to the field over the past decade. I believe that my expertise and experience make me qualified to conduct these research project successfully.
Our laboratory discovered a novel antiproliferative factor (APF) glycopeptide that is made specifically by bladder epithelial cells from IC/PBS patients and whose biological activity is detectable in urine from approximately 95% of IC/PBS patients. APF induces changes in expression of certain epithelial cell proteins, decreases tight junction formation, increases paracellular permeability, and profoundly inhibits the proliferation of normal bladder epithelial cells in vitro; these same changes have been confirmed in cells grown from IC/PBS patient biopsies as compared to normal controls, suggesting that APF may contribute to the pathogenesis of IC/PBS. Inhibition of APF activity or production may therefore be useful for the development of therapies for this disorder.
Keay S, Szekely Z, Conrads TP, Veenstra TD, Barchi JJ, Zhang C-O, Koch KR, Michejda CJ. An antiproliferative factor from interstitial cystitis patients is a frizzled 8 protein-relater sialoglycopeptide. PNAS, 2004,Vol 101, no 32, 11803- 11808.
Keay S, Warren JW, Zhang C-O, Tu LM, Gordon DA Whitmore KE. Antiprofilerative activity is present in bladder but not renal pelvic urine from interstitial cystitis patients. J of Urology, Vol. 162, 1487-1489, 1999.
. My research has been highly focused on investigating the pathogenesis of interstitial cystitis, especially the cellular and molecular mechanisms through which human bladder epithelial cell proliferation and interaction are regulated under biological and pathological conditions. I have made a great contribution to our understanding of bladder mucosal inflammation and epithelial barrier function. The project utilizes state of the art cellular and molecular methodology and the progress of my works over the last 20 years is most impressive not only in the quantity but also more importantly in quality. During this period, I have co-authored many papers that have been published or accepted by prestigious Journals. my productivity and specific expertise in cellular and molecular epithelial biology makes it clear that I have become an extremely valuable investigator in our uro-epithelial biology research group.
no clinical speciality.