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Shiladitya DasSarma, PhD

Academic Title:

Professor

Primary Appointment:

Microbiology and Immunology

Location:

Columbus Center, 5047

Phone (Primary):

410-234-8847

Phone (Secondary):

410-234-8861

Fax:

410-234-8896

Education and Training

Indiana University, Chemistry, B.S. (Honors)

Massachusetts Institute of Technology, Biochemistry, Ph.D.

Massachusetts General Hospital, Harvard Medical School, Postdoctoral Fellow

Biosketch

Dr. DasSarma is a pioneering microbiologist well-known for his contributions to the genomics, molecular biology, and biotechnology of Archaea and the field of Astrobiology. Prior to joining the Institute of Marine and Environmental Technology, University of Maryland School of Medicine, he was a Professor in the University of Massachusetts Amherst and the University of Maryland Biotechnology Institute. He has published over 150 scientific papers over his career and mentored many students. He founded the UMB GPILS genomics track and is the UMB institutional representative for the system-wide MEES graduate program.

Please visit the DasSarma Laboratory to learn more.

Research/Clinical Keywords

extremophiles, haloarchaea, microbial genomics, purple membrane, gas vesicle nanoparticles, vaccines and therapeutics

Highlighted Publications

DasSarma, S. 2006. Extreme Halophiles Are Models for Astrobiology. Microbe 1:120-126.

DasSarma, S. 2007. Extreme Microbes: The Salty Side of Life. American Scientist 95:224-231.

DasSarma, S., Coker, J. A. and DasSarma, P. Archaea (overview).  2009. The Desk Encyclopedia of Microbiology, pp. 118-139, Oxford: Elsevier.

Slonczewski, J. L., Coker, J. A. and DasSarma, S.  2010.  Microbial Growth with Multiple Stressors.  Microbe  5: 110-116.

DasSarma, S. and DasSarma, P. 2012. HalophilesEncyclopedia of Life Sciences, Wiley.

DasSarma, S. and DasSarma, P. 2015. Halophiles and their enzymes: negativity put to good use. Current Opinion in Microbiology 25:120–126.

DasSarma, S. and DasSarma, P. 2015. Gas Vesicle Nanoparticles for Antigen Display. Vaccines 3:686-702.

Research Interests

Please see the DaSarma Lab to learn about Dr. DaSarma's research interests.

Links of Interest

DasSarma Lab Group

The DasSarma laboratory was established in 1986, soon after the discovery of the Archaea. Since then, we have been studying these novel microbes and their mechanisms of adaptation to challenging habitats, including extreme environments and the human microbiome. We use a combination of genetics, genomics, bioinformatics, transcriptomics, and other approaches to address problems of fundamental importance. Our favorite model organism is Halobacterium sp. NRC-1, a salt-loving, radiation-resistant, and desiccation-tolerant archaeon that is easy to culture in the lab and genetically tractable.

We led the sequencing project on Halobacterium (with an international consortium of 12 laboratories), which was published in the October 2000 issue of the Proceedings of the National Academy of Sciences USA. The NRC-1 genome consists of a 2 Mbp circular chromosome and two large plasmids, pNRC100 (191 kbp) and pNRC200 (365 kbp).

We have since studied many of the ca. 2,500 genes in the genome and pursued translational research through genetic engineering and biotechnology. For example, the gas vesicle gene cluster of Halobacterium, which has been of long term interest, is bioengineerable, and is being used for therapeutic and antigenic protein delivery and vaccine development.

Our studies of microaerobically induced genes led to the discovery of novel regulators, such as the bacterio-opsin activator (Bat) and DMSO reductase regulator (DmsR). Gene regulation studies in our lab have also led to the discovery that expanded families of eukaryotic-type transcription factors (TBP and TFB) coordinate promoter selection and expression of different classes of genes under different growth conditions.

Another interest of the lab concerns DNA replication and repair in Halobacterium, which employs both eukaryotic-type proteins (origin binding and single-stranded DNA binding, Orc and RPA) and bacterial-type proteins (nucleotide excision repair, UvrABC). These and other current studies in our laboratory are helping to better understand the strategies for microbial survival and adaptation to develop applications in medicine and the environment.

Visit the DasSarma Laboratory