Personal HistoryEducation • Amherst College, Amherst, MA: BA (magna cum laude), Biology/Independent Study, 1970 Employment History Honors • National Merit Finalist; Westinghouse & Ford Foundation Science Awards (honorable mention); National Honor Society & American Legion Scholarships
Active Research Grants:
P01CA70970 Sharkis (PI) 6/30/98-1/31/13 NIH/NCI Hematopoietic Stem Cells for Transplantation The major goals of this Program Project are to better understand the pathophysiology of normal hematopoietic stem cells (HSCs) and abnormal HSCs in human diseases. Dr. Civin is budgeted to receive effort support and leads Project 4 entitled “MicroRNA targeting of normal and leukemia stem-progenitor cells”. The specific aims of Core A are to provide central leadership, administrative management, and mentoring. The goals of Project 4 are to (1) quantify microRNA and mRNA expression in acute myeloid leukemia (AML) and acute lymphoid leukemia (ALL) stem cells vs the bulk population of AML and ALL blast cells, respectively, to predict which microRNAs may control leukemia stem cells (LSCs), and (2) determine the cellular and molecular mechanisms by which selected microRNAs affect the biology of LSCs vs the bulk AML and ALL cells. Role: Co- Principal Investigator T32CA60441 Small (PI) 9/30/93-5/31/13 NIH/NCI Lab Research Training in Pediatric Oncology-Hematology The major goals of this project are to provide postdoctoral lab research training for MDs and PhDs on topics highly related to childhood cancer and blood diseases (10 slots/yr). C. Civin receives no salary support. Role: Investigator No number Civin (PI) 10/1/03-9/30/13 National Foundation for Cancer Research NFCR Fellow Award This grant provides funds to support general laboratory infrastructure and specific research that is not specifically funded by any other source. Role: Principal Investigator No number Civin (PI) 7/1/07-6/30/09 Samuel Waxman Cancer Research Foundation MicroRNAs regulating acute leukemias The major goals of this project are to elucidate the effects of selected hematopoietic stem-progenitor cell (HSPC)-expressed microRNAs, especially mir-155, on human leukemia stem cells (LSCs) and to determine the molecular mechanisms of these cellular microRNA effects. Role: Principal Investigator LLS 2007 Translational Grant 6082-08 Civin (PI) 10/1/07-09/30/10 Leukemia & Lymphoma Society CD22 Immunotoxins for Acute Lymphoblastic Leukemia The major goals of this project are to translationally develop CD22 immunotoxins for B-precursor ALL by (1) preclinically assessing the efficacy of the “HA22” CD22 immunotoxin against the ALL stem cell, (2) preclinically exploring potential synergies of HA22 with standard chemotherapeutic agents, and (3) advancing the most promising approaches to our human Phase II clinical trials. LLS Translational Grants may be competitively renewed for years 4-5 of funding, if there is a clinical trial ongoing (as there is in this case). Role: Principal Investigator 2007-MSCRFII-0114 Civin (PI) 10/1/07-9/30/10 Maryland Stem Cell Research Fund MicroRNA Regulation of Adult and Embryonic Human Hematopoietic Development The major goals of this project are (1) to expand our microRNA profiling to highly purified subsets of primary adult human hematopoietic stem-progenitor cells (HSPCs) and human embryonic stem cell (hESC)-derived embryonic hematopoietic cells, (2) to determine the stages of hematopoiesis at which mir-155 blocks normal hematopoiesis, and (3) to study the functional effects and molecular mechanism of mir-155 on embryonic hematopoiesis generated from hESCs. This project involves profiling normal primary and hESC-derived HSPC subsets. The ultimate goal of this Maryland Stem Cell Research Fund project is to extend our understanding of the developmental origin of hematopoiesis, and is not focused on erythropoiesis. Role: Principal Investigator 2007-MSCRFII-0090 Zambidis (PI) 10/1/07-9/30/10 Maryland Stem Cell Research Fund Human Embryonic Stem Cell Models of Normal and Leukemic HSC The major goals of this project are to expand the novel human embryonic stem cell (hESC)-based hematopoietic model originally developed by this PI, and now extend toward studying the detailed developmental origins of normal and leukemic human HSCs. Role: Investigator 2007-MSCRFE-0089 Chandrasegaran (PI) 10/1/07-9/30/09 Maryland Stem Cell Research Fund Targeted Engineering of the Human Genome in Stem Cells The major goal of this project is to develop zinc-finger nuclease technologies to genetically modify human adult or embryonic stem cells. Role: Investigator R01GM077291 Chandrasegaran (PI) 8/1/07-7/31/12 NIH/NIGMS Developing ZFNs as Molecular Tools for Targeted Integration The major goal of this project is to develop zinc-finger nucleases as reagents for site-specific modification of mammalian cells including human cells. Role: Investigator R01DK080750 Civin (PI) 4/1/08-3/31/11 NIH/NIDDK MicroRNAs regulating erythroid development In this project, we seek to extensively profile microRNA expression during the early stages of human and mouse erythropoiesis. We will determine if selected erythroid-expressed microRNAs (“E-microRNAs”) functionally affect erythropoiesis. Using erythroid cell lines and primary cells, we will then identify proteins whose synthesis is inhibited by each E-microRNA that affects erythropoiesis. Identifying the molecular mechanisms of the erythropoietic effects of these E-microRNAs may lead to novel means to expand and control erythroid development for research and potential clinical applications. Role: Principal Investigator Publications
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