Diagnostic Radiology and Nuclear Medicine
670 W. Baltimore Street, Baltimore, MD 21201,
Education and Training
2014 - 2018 Research Associate, Memorial Sloan Kettering Cancer Center, NY
2012 - 2014 Postdoctoral Research Fellow, Stanford University, CA
2007 - 2011 PhD Biochemistry, Christ’s College, University of Cambridge, UK
2004 - 2007 BA (Hons) Natural Science, Christ’s College, University of Cambridge, UK
My lab is interested in the intersection of cancer signaling pathways and metabolism, with the ultimate aim to translate these findings for novel metabolic imaging applications.
Hyperpolarized magnetic resonance spectroscopy (HP-MRS) is a unqiue metabolic imaging platform that enables real-time, non-invasive detection of metabolic flux in living cells and model organisms. I have pioneered the development of reporter genes for HP-MRS by genetically modifying cells to over-express bacterial and mammalian enzymes. Leveraging my expertise in molecular biology, I am now investigating oncogenic signaling and metabolic pathways.
Specifically, I am interested in using targeted therapeutics to inhibit specific pathways mutated in cancers and investigating the metabolic consequence of inhibition. Using this information, we will design novel metabolic imaging probes for both diagnosing/staging cancer as well as assessing treatment response and development of resistance.
Metabolism, Hyperpolarized, Magnetic resonance imaging, Magnetic resonance spectroscopy, Cancer, Gene reporter, Biochemistry
a) Patrick, P.S., Hammersley, J., Loizou, L., Kettunen, M.I., Rodrigues, T.B., Hu, D.E., Tee. S.S., Hesketh, R., Lyons, S.K., Soloviev, D., Lewis, D.Y., Aime, S., Fulton, S.M., Brindle, K.M., Dual-modality gene reporter for in vivo imaging, Proc. Natl. Acad. Sci. (2014)
b) Ye, D., Shuhendler, AJ., Cui, L., Tong, L., Tee, S.S., Tikhomirov, G., Felsher, D.W., Rao, J., Bioorthogonal cyclization-mediated in situ self-assembly of small-molecule probes for imaging caspase activity in vivo. Nat. Chem. (2014)
c) Dzien, P.,* Tee, S.S., * Kettunen, M.I., Lyons, S.K., Larkin T., Hu, D.E., Rodrigues, T.B., Serrao, E., Marco-Rius, I., Mannion, E., D‘Santos, P., Kennedy, B.W., Brindle K.M., 13C magnetic resonance spectroscopy measurements with hyperpolarized [1-13C] pyruvate can be used to detect the expression of transgenic pyruvate decarboxylase activity in vivo, Magn. Reson. Med. (2015)
d) Digialleonardo, V., Tee, S.S., Aldeborgh, H., Eskandari, E., Jeong, S., Poot, A., Truong, S., Alvarez, J.A., Keshari, K.R., High-throughput indirect quantitation of 13C enriched metabolites using 1H NMR, Anal. Chem. (2016)
e) Tee, S.S., Digialleonardo, V., Eskandari, E., Jeong, S., Poot, A., Truong, S., Alvarez, J.A., Aldeborgh, H., Keshari, K.R., Sampling Hyperpolarized Molecules Utilizing a 1 Tesla Permanent Magnet. Sci. Rep. (2016)
f) Tee, S.S., Park, J., Billingsley, K., Josan, S., Hurd, R., Spielman, D., 2-deoxyglucose sensitizes cancer cells to PKM2 activation, Oncotarget (2017)
g) Tee, S.S., Suster, I., Truong, S., Digialleonardo, V., Eskandari, R., Jeong, S., Alvarez, J.A., Aldeborgh, H., Keshari, K.R., Targeted AKT inhibition in prostate cancer cells and spheroids reduces aerobic glycolysis and conversion of hyperpolarized [1-13C] pyruvate to [1-13C] lactate. Mol. Cancer Res. (2018)
h) Cell Metab. (2019)Hyperpolarized MRI of Human Prostate Cancer Reveals Increased Lactate with Tumor Grade Driven by Monocarboxylate Transporter 1.