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Avneesh Kumar Singh, PhD

Academic Title:

Assistant Professor

Primary Appointment:

Surgery

Location:

MSTF,Rm 434F, 10 S Pine Street

Phone (Primary):

410-706-0320

Fax:

410-706-0321

Education and Training

B.Sc. (Hons.), Zoology, AMU, Aligarh, UP, 202001, INDIA

M.Sc., Zoology, AMU, Aligarh, UP, 202001, INDIA

Ph.D., Zoology (Immunogenetics); SGPGIMS, (LU), Lucknow, UP, INDIA

 

Postdoctoral Training

Dept. of Microbiology & Immunology, Vanderbilt University, Nashville, TN, USA
(Mentor or PI: Dr. Luc Van Kaer, PI, Howard Hughes Medical Institute)

 

PROFESSIONAL EXPERIENCE

2005-2006 Research Scientist [C], Transplantation Biology Branch, NIDDK/NIH Bethesda, MD @ Kelly Services at NIH

2006-2006 Scientist, Central Drug Research Institute, Lucknow, UP, India

2006- 2017 Scientist, Suburban Hospital, Johns Hopkins Medicine, Bethesda, MD

2007- 2017 Co-Investigator in Cardiothoracic Surgery Research Program, NHLBI/NIH, Bethesda, MD

2016-2017 Assistant Professor (PT) Department of Surgery, Johns Hopkins Medicine, Baltimore, MD

Biosketch

Received Dr. K.S. Krishnan Research (DAE) Fellowship from Govt. of India to pursue the doctorate degree in life science and investigated the effect of non-immunological and immunological factors (e.g. HLA, alloantibodies, MLR-Bf etc.) on kidney allograft rejection and survival.

During postdoctoral Training from Vanderbilt University, Nashville, TN,  investigated the role of natural killer T (NK T) cells in autoimmune disease.

Later moved to Cardiothoracic Surgery Research Program, NIH heart center at Suburban hospital, sponsored by NHLBI/NIH, Bethesda as Research Scientist to do research on the pig to baboon preclinical cardiac xenotransplantation model.

The focus of my research is to identify an ideal donor from genetically modified pig and optimize the immunosuppression for cardiac xenotransplantation to benefit the heart failure patients for their better life.

Contributions to Science

  • Successfully achieved more than two-year cardiac xenograft survival from genetically engineered pigs along with modified immunosuppression
  • Successfully purified and successfully expanded baboon naturally occurring CD4+CD25+ Treg cells using irradiated pig PBMC and IL-2, and have demonstrated suppression of autologous effector CD4+CD25neg T-cell and B cells proliferation
  • Demonstrated immunoregulatory role of natural killer T (NKT) cells in autoimmune diseases
  • Reported that the activation of NKT cells by its ligand (i.e. α -galactosylceramide (α-GalCer), isolated from marine sponges) prevents experimental autoimmune encephalomyelitis (EAE), an experimental model of human multiple sclerosis and murine systemic lupus Erythematosus (SLE) autoimmune disease induced by pristine
  • Analyzed the early response of NKT cells to glycolipid antigens and bacterial infection by using specific reagents for tracking these cells

Highlighted Publications

Singh AK, Corcoran PC, Lewis BG, Thomas, ML, Eckhaus M, Ayares D Horvath KA and Mohiuddin MM; Cardiac xenotransplantation: CD4+CD25HiFoxP3+ T regulatory cells in long-term cardiac xenograft survivors; Xenotransplantation. 2017; Accepted


Mohiuddin MM, Singh AK, Corcoran PC, Thomas, III, ML, Clark T , Lewis BG, Hoyt RG, Eckhaus M, Pierson III RN, Belli AJ, Wolf E, Klymiuk N, Phelps C, Reimann KA, Ayares D and Horvath KA; Chimeric 2C10R4 anti-CD40 antibody therapy is critical for long-term survival of GTKO.hCD46.hTBM cardiac xenograft; Nature Communications; 2016 Apr 5;7:11138. DOI: 10.1038/ncomms11138: PMID:27045379


Mohiuddin MM, Singh AK, Corcoran PC, Hoyt RF, Thomas ML 3rd, Lewis BG, Eckhaus M, Reimann KA, Klymiuk N, Wolf E, Ayares D, Horvath KA; One-year heterotopic cardiac xenograft survival in a pig to baboon model; American J Transplant. 2014 Feb; 14(2): 488-9.DOI: 10.1111/ajt.12562. Epub 2013 Dec 11; PMID: 24330419


Singh AK, Seavey C, Horvath KA and Mohiuddin MM: Ex−Vivo Expanded CD4+CD25+Treg Cells Suppress T and B Immune Response; Xenotransplantation; 2012 Mar; 19(2): 102-11; PMID 22497512


Parekh, VV, Wilson MT, Olivares-Villagómez D, Singh AK, Wu L, Wang CR, Joyce S, and Van Kaer L; Glycolipid antigen induces long-term NKT cell anergy in mice. Journal of Clinical Investigation 2005 Sep; 115(9): 2572


Singh AK, Wilson MT, Hong S, Olivares-Villagómez D, Du C, Stanic AK, Joyce S, Sriram S, Koezuka Y, and Van Kaer L: Natural Killer T Cell Activation Protects Mice against Experimental Autoimmune Encephalomyelitis; J EXP MED 194 (12): 1801-1811 DEC 17 2001

Additional Publication Citations

 

  • Cooper, Wijkstrom, Hariharan et al. 2017 Transplantation 101, 1551-1558,
  • Mohiuddin, Singh, Corcoran et al. 2016 Nat Commun 7, 11138,
  • Azimzadeh, Kelishadi, Ezzelarab et al. 2015 Xenotransplantation 22, 310-316,
  • Zhou, Singh, Hoyt et al. 2014 J Thorac Cardiovasc Surg 148, 1131-1137; discussion 1117,
  • Wang, Zhou, Andreyev Singh et al. 2014 Exp Cell Res 323, 56-65,
  • Mohiuddin, Singh, Corcoran et al. 2014 Am J Transplant 14, 488-489,
  • Mohiuddin, Singh, Corcoran et al. 2014 Xenotransplantation 21, 35-45,
  • Mohiuddin, Singh, Corcoran et al. 2014 J Thorac Cardiovasc Surg 148, 1106-1113; discussion 1113-1104,
  • Singh, Seavey, Horvath et al. 2012 Xenotransplantation 19, 102-111,
  • Mohiuddin, Corcoran, Singh et al. 2012 Am J Transplant 12, 763-771,
  • Wang, Zhou, Seavey Singh et al. 2010 Stem Cell Res 4, 117-128,
  • Horvath, Corcoran, Singh et al. 2010 Transplant Proc 42, 2152-2155,
  • Corcoran, Horvath, Singh et al. 2010 Transplant Proc 42, 2149-2151,
  • Singh, Horvath and Mohiuddin 2009 Transplant Proc 41, 418-421,
  • Porter, Horvath-Arcidiacono, Singh et al. 2007 Xenotransplantation 14, 298-308,
  • Singh, Yang, Parekh et al. 2005 Eur J Immunol 35, 1143-1154,
  • Parekh, Wilson, Olivares-Villagomez, Singh et al. 2005 J Clin Invest 115, 2572-2583,
  • Parekh, Singh, Wilson et al. 2004 J Immunol 173, 3693-3706,
  • Yang, Singh, Wilson et al. 2003 J Immunol 171, 2142-2153,
  • Wilson, Johansson, Olivares-Villagomez, Singh  et al. 2003 Proc Natl Acad Sci U S A 100, 10913-10918,
  • Wilson, Singh and Van Kaer 2002 Trends Mol Med 8, 225-231,
  • Singh, Wilson, Hong et al. 2001 J Exp Med 194, 1801-1811

Published abstracts:

  • Chan, Singh, Thomas et al. 2016 Journal of Heart and Lung Transplantation 35, S47-S48,
  • Chan, Singh, Thomas et al. 2016 Circulation 134,
  • Mohiuddin, Singh, Corcoran et al. 2014 American Journal of Transplantation 14, 488-489,
  • Singh, Seavey, Horvath et al. 2012 Xenotransplantation 19, 102-111,
  • Singh, Seavey, Corcoran et al. 2012 Transplantation 94, 68-68,
  • Mohiuddin, Singh, Corcoran et al. 2012 American Journal of Transplantation 12, 530-531,
  • Mohiuddin, Singh, Corcoran et al. 2012 Transplantation 94, 70-70,
  • Singh, Seavey, Horvath et al. 2010 American Journal of Transplantation 10, 21-21,
  • Singh, Seavey, Horvath et al. 2010 American Journal of Transplantation 10, 298-298,
  • Mohiuddin, Corcoran, Singh et al. 2010 American Journal of Transplantation 10, 186-186,
  • Horvath, Corcoran, Singh et al. 2010 American Journal of Transplantation 10, 299-299,
  • Corcoran, Horvath, Singh et al. 2010 American Journal of Transplantation 10, 300-300,
  • Mohiuddin, Singh, Seavey et al. 2009 Xenotransplantation 16, 362-363,
  • Mohiuddin, Singh, Corcoran et al. 2009 Xenotransplantation 16, 357-357,
  • Horvath, Corcoran, Singh et al. 2009 Xenotransplantation 16, 380-380,
  • Corcoran, Horvath, Singh et al. 2009 Xenotransplantation 16, 429-429,
  • Azimzadeh, Kelishadi, Ezzelarab et al. 2009 Xenotransplantation 16, 356-356,
  • Singh, Horvath and Mohiuddin 2008 American Journal of Transplantation 8, 562-562,
  • Wilson, Johansson, Olivares-Villagomez et al. 2003 Faseb Journal 17, C82-C83,
  • Singh, Singh, Wang et al. 2003 Journal of the American Society of Nephrology 14, 383a-383a,
  • Singh, Yang, Wilson et al. 2003 Faseb Journal 17, C279-C279,
  • Yang, Singh, Hong et al. 2002 Faseb Journal 16, A326-A326,
  • Singh, Wilson, Hong et al. 2002 Faseb Journal 16, A1043-A104,
  • Singh, Hong, Du et al. 2001 Faseb Journal 15, A1212-A1212 

 

Awards and Affiliations

  • Young Investigator Travel Grant for attending International Xenotransplantation Meeting in Osaka, Japan (2013)
  • The Sidney P. Colowick Award for outstanding postdoctoral research achievements from Vanderbilt University, Nashville, Tennessee (2002)
  • Dr. K. S. Krishnan Research (DAE) Fellowship for pursuing Ph.D. in life sciences (1993)
  • National Merit Scholarship in High School (1985)