Academic Title:
Research Associate
Primary Appointment:
Microbiology and Immunology
Location:
Health Sciences Facility III, 670 West Baltimore St, Baltimore 21201
Phone (Primary):
410-706-2376
Education and Training
B.S., 2004, University of California, Davis
Ph.D., 2009, Georgia Institute of Technology
Biosketch
During my Ph.D. I studied microbial ecology and the contribution of plasmids and phages to the genetic diversity of bacteria in coastal marine environments. I developed an interest in learning bioinformatics techniques that lead me to do my postdoctoral training at the Institute for Genome Sciences with Dr. David Rasko. Since starting at IGS I have been using comparative genomics and transcriptomics to investigate the genomic diversity of pathogenic E. coli. I have also been investigating the antibiotic resistance mechanisms of Klebsiella and Acinetobacter species. As a Research Associate at IGS I am interested in the genetic diversity of plasmids and their role in the development of disease and antibiotic resistance of enterics.
Research/Clinical Keywords
E. coli, genomics, evolution, antibiotic resistance
Highlighted Publications
- Hazen, T.H., J.W. Sahl, M.S. Donnenberg, C.M. Fraser, F. Scheutz, and D.A. Rasko. 2013. Refining the pathovar paradigm via phylogenomics of the attaching and effacing Escherichia coli. Proc. Natl. Acad. Sci. USA, 110:12810-12815. (PMID: 23858472)
- Subashchandrabose, S., T.H. Hazen, A.R. Brumbaugh, S.D. Himpsl, S.N. Smith, R.D. Ernst, D.A. Rasko, and H. Mobley. 2014. Human-specific induction of Escherichia coli fitness genes during clinical urinary tract infection. Proc. Natl. Acad. Sci. USA 111: 18327-32. (PMID: 25489107)
- Hazen, T.H., L. Zhao, M.A. Boutin, G. Robinson, A.D. Harris, D.A. Rasko, and J.K. Johnson. 2014. Comparative genomics of an IncA/C multidrug resistance plasmid from Escherichia coli and Klebsiella species isolated from ICU patients: the utility of whole genome sequencing in healthcare settings. Antimicrob. Agents Chemother. 58: 4814-4825. (PMID: 24914121)
- Hazen, T.H., J.B. Kaper, J.P. Nataro, and D.A. Rasko. 2015. Comparative genomics provides insight into the diversity of the attaching and effacing Escherichia coli (AEEC) virulence plasmids. Infect. Immun. 83:4103-4117. (PMID: 26238712)
- Hazen, T.H., M.S. Donnenberg,S. Panchalingam, M. Antonio, A. Hossain, I. Mandomando, J.B. Ochieng, T. Ramamurthy, B. Tamboura, S. Qureshi, F. Quadri, A. Zaidi, K.L. Kotloff, M.M. Levine, E.M. Barry, J.B. Kaper, D.A. Rasko, and J.P. Nataro. 2016. Genomic diversity of EPEC associated with clinical presentations of differing severity. Nat. Microbiol. (PMID: 27571975)
Additional Publication Citations
- Hazen, T.H., D. Wu, J.A. Eisen, and P.A. Sobecky. 2007. Sequence characterization and comparative analysis of three plasmids isolated from environmental Vibrio spp. Appl. Env. Microbiol. 73:7703-7710. (PMID: 17921277)
- Criminger, J.D., T.H. Hazen, P.A. Sobecky, and C.R. Lovell. 2007. Nitrogen fixation by Vibrio parahaemolyticus and its implications for a new ecological niche. Appl. Env. Microbiol.73:5959-5961. (PMID: 17675440)
- Hazen, T.H., K.D. Kennedy, S. Chen, S.V. Yi, and P.A. Sobecky. 2009. Inactivation of mismatch repair increases the diversity of Vibrio parahaemolyticus. Environ. Microbiol. 11:1254-1266. (PMID: 19161434)
- Sobecky, P.A., and T.H. Hazen. 2009. Horizontal gene transfer and mobile genetic elements in marine systems. Horizontal gene transfer: genomes in flux. Humana Press. Eds. M.B. Gogarten et al. 532:435-453. (PMID: 19271200)
- Hazen, T.H., R.J. Martinez, Y. Chen, P.C. Lafon, N.M. Garrett, M.B. Parsons, C.A. Bopp, M.C. Sullards, and P.A. Sobecky. 2010. Rapid identification of Vibrio parahaemolyticus by whole-cell matrix assisted laser desorption ionization-time of flight mass spectrometry. Appl. Environ. Microbiol.75:6745-6756. (PMID: 19749061)
- Hazen, T.H., L. Pan, J.D. Gu, and P.A. Sobecky. 2010. Contribution of mobile genetic elements to the ecology and evolution of Vibrios. FEMS Microbiol. Ecol.Review. 74:485-499. (PMID: 20662928)
- Hazen, T.H., G. Robinson, A.D. Harris, D.A. Rasko, J.K. Johnson. 2012. Genome sequence of Klebsiella oxytoca 11492-1, a nosocomial isolate possessing a FOX-5 AmpC β-lactamase. J. Bacteriol. 194:3028-3029. (PMID: 22582383)
- Hazen, T.H., J.W. Sahl, J. Redman, C. Morris, S. Daugherty, M. Chibucos, N. Sengamalay, C. Fraser-Liggett, H. Steinsland, T. Whittam, B. Whittam, S. Manning, and D.A. Rasko. 2012. Draft genome sequence of diarrheagenic Escherichia coli collection. J. Bacteriol. 194:3026-3027. (PMID: 22582382)
- Subashchandrabose, S., T.H. Hazen, D.A. Rasko, and H. Mobley. 2013. Draft genome sequences of five recent human uropathogenic Escherichia coli isolates. Pathogens Dis. (PMID: 23821517)
- Nisa, S., T.H. Hazen, L. Assatourian, J.P. Nougayrède, D.A. Rasko, and M.S. Donnenberg. 2013. In vitro evolution of an archetypal enteropathogenic Escherichia coli strain. J. Bacteriol. 195:4476-4483. (PMID: 23913321)
- Hazen, T.H., J.W. Sahl, C.M. Fraser, M.S. Donnenberg, F. Scheutz, and D.A. Rasko. 2013. Draft genome sequences of three O157 enteropathogenic Escherichia coli isolates. Genome Announc. (PMID: 23868135)
- Hazen, T.H., J.W. Sahl, M.S. Donnenberg, C.M. Fraser, F. Scheutz, and D.A. Rasko. 2013. Refining the pathovar paradigm via phylogenomics of the attaching and effacing Escherichia coli. Proc. Natl. Acad. Sci. USA, 110:12810-12815. (PMID: 23858472).
- Levine, J.A., A.M. Hansen, J.M. Michalski, T.H. Hazen, D.A. Rasko, and J. Kaper. 2014. H-NST induces LEE expression and the formation of attaching and effacing lesions in enterohemorrhagic Escherichia coli. Plos One, 9(1):e86618. (PMID: 24466172)
- Sanjar, F., T. Hazen, S. Shah, S. Koenig, S. Agrawal, S. Daugherty, L. Sadzewicz, L. Tallon, M. Mammel, P. Feng, R. Soderlund, P. Tarr, C. DebRoy, E. Dudley, T. Cebula, J. Ravel, C. Fraser, D. Rasko, and M. Eppinger. 2014. Genome sequence of Escherichia coli O157:H7 strain 2886-75 associated with the first reported case of human infection in the United States. Genome Announc. 2: e01120-13. (PMID: 24407635)
- Hazen, T.H., L. Zhao, J.W. Sahl, G. Robinson, A.D. Harris, D.A. Rasko, and J.K. Johnson. 2014. Characterization of Klebsiella sp. 10982, a colonizer of humans that contains novel antibiotic resistance alleles and exhibits genetic similarities to plant and clinical Klebsiella isolates. Antimicrob. Agents Chemother. 58: 1879-88. (PMID: 24395222)
- Hazen, T.H., L. Zhao, M.A. Boutin, G. Robinson, A.D. Harris, D.A. Rasko, and J.K. Johnson. 2014. Comparative genomics of an IncA/C multidrug resistance plasmid from Escherichia coli and Klebsiellaspecies isolated from ICU patients: the utility of whole genome sequencing in healthcare settings. Antimicrob. Agents Chemother. 58: 4814-4825. (PMID: 24914121)
- Hazen, T.H., M.S. Humphrys, B. Ochieng, M. Parsons, C.A. Bopp, E. Mintz, C. O’Reilly, and D.A. Rasko. 2014. Draft genome sequences of nine enteropathogenic Escherichia coli from Kenya. Genome Announc. e00582-14 (PMID: 24926061)
- Sadiq, S.M., T.H. Hazen, D.A. Rasko, M. Eppinger. 2014. EHEC Genomics: Past, present and future. Microbiol. Spectr. (PMID: 26104203)
- Doi, Y., T.H. Hazen, M. Boitano, Y-C. Tsai, T.A. Clark, J. Korlach, D.A. Rasko. 2014. Whole genome assembly of a Klebsiella pneumoniae strain co-producing NDM-1 and OXA-232 carbapenemases using single-molecule, real-time sequencing. Antimicrob. Agents Chemother. 58: 5947-53. (PMID: 25070096)
- Subashchandrabose, S., T.H. Hazen, A.R. Brumbaugh, S.D. Himpsl, S.N. Smith, R.D. Ernst, D.A. Rasko, and H. Mobley. 2014. Human-specific induction of Escherichia coli fitness genes during clinical urinary tract infection. Proc. Natl. Acad. Sci. USA 111: 18327-32. (PMID: 25489107)
- Brumbaugh, A., S. Smith, S. Subashchandrabose, S. Himpsl, T. Hazen, D. Rasko, and H. Mobley. 2015. Blocking yersiniabactin import attenuates extraintestinal pathogenic Escherichia coli in cystitis and pyelonephritis and represents a novel target to prevent urinary tract infection. Infect. Immun. 83: 1443-50. (PMID: 25624354)
- Hazen, T.H., P.C. Lafon, N.M. Garrett, T.M. Lowe, D.J. Silberger, L.A. Rowe, M. Frace, M.B. Parsons, C.A. Bopp, D.A. Rasko, and P.A. Sobecky. 2015. Insights into the environmental reservoir of pathogenic Vibrio parahaemolyticus using comparative genomics. Front. Microbiol. (PMID: 25852665)
- Donnenberg, M., T.H. Hazen, T.H. Farag, S. Panchalingam, M. Antonio, A. Hossain, I. Mandomando, J.B. Ochieng, T. Ramamurthy, B. Tamboura, A. Zaidi, M.M. Levine, K. Kotloff, D.A. Rasko, and J.P. Nataro. 2015. Bacterial factors associated with lethal outcome of enteropathogenic Escherichia coli infection: genomic case-control studies. Submitted to Plos Negl. Trop. Dis. (PMID: 25978422)
- Sanjar, F., B. Rusconi, T. Hazen, S. Eppinger, M. Mammel, P. Feng, D. Rasko, and M. Eppinger. 2015. Characterization of the pathogenome and phylogenomic classification of enteropathogenic Escherichia coli of the O157:non-H7 serotypes. Pathog. Dis. (PMID: 25962987)
- Hazen, T.H., S.C. Daugherty, A.C. Shetty, A.A. Mahurkar, O. White, J.B. Kaper, and D.A. Rasko. 2015. RNA-Seq analysis of isolate- and growth phase-specific differences in the global transcriptomes of enteropathogenic Escherichia coli prototype strains. Front. Microbiol. (PMID: 26124752)
- Hazen, T.H., J.B. Kaper, J.P. Nataro, and D.A. Rasko. 2015. Comparative genomics provides insight into the diversity of the attaching and effacing Escherichia coli (AEEC) virulence plasmids. Infect. Immun. 83:4103-4117. (PMID: 26238712)
- Kessler, R., S. Nisa, T.H. Hazen, A. Horneman, A. Amoroso, D.A. Rasko, and M.S. Donnenberg. 2015. Diarrhea, bacteremia and multiorgan dysfunction due to an extraintestinal pathogenic Escherichia coli strain with divergent enteropathogenic E. coli genes. Pathog. Dis. (PMID: 26410828)
- Hazen, T.H., M.S. Donnenberg,S. Panchalingam, M. Antonio, A. Hossain, I. Mandomando, J.B. Ochieng, T. Ramamurthy, B. Tamboura, S. Qureshi, F. Quadri, A. Zaidi, K.L. Kotloff, M.M. Levine, E.M. Barry, J.B. Kaper, D.A. Rasko, and J.P. Nataro. 2016. Genomic diversity of EPECassociated with clinical presentations of differing severity. Nat. Microbiol. (PMID: 27571975)
- Kania, D.A., T.H. Hazen, A. Hossain, J.P. Nataro, and D.A. Rasko. 2016. Genome dynamics of Shigella boydii. Pathog. Dis. (PMID: 27056949)
- Hazen, T.H., S.R. Leonard, K.A. Lampel, D.W. Lacher, A.T. Maurelli, and D.A. Rasko. 2016. Investigating the relatedness of enteroinvasive Escherichia coli to other E. coli and Shigella using comparative genomics. Infect. Immun. 84:2362-71 (PMID: 27271741)
- Chibucos, M.C., S. Soliman, T. Ghebremarimam, H. Lee, S. Daugherty, J. Orvis, A.C. Shetty, J. Crabtree, T.H. Hazen, K.A. Etienne, P. Kumari, T.D. O’Connor, D.A. Rasko, C.M. Fraser, S.R. Lockhart, C.D. Skory, A.S. Ibrahim, and V.M. Bruno. 2016. An integrated genomic and transcriptomic survey of mucormycosis-causing fungi. Nat. Commun. (PMID: 27447865)