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David A. Rasko, PhD

Academic Title:

Professor

Primary Appointment:

Microbiology and Immunology

Additional Title:

Professor, Institute for Genome Sciences

Location:

BioPark II, 801 W. Baltimore St., Suite 619

Phone (Primary):

(410) 706-6774

Fax:

(410) 706-1482

Education and Training

Education

 1995                B.Sc.               Specialization in Microbiology, with Distinction

                                               University of Alberta, Edmonton, Alberta, Canada

2000                Ph.D.               Department of Medical Microbiology and Immunology

                                               University of Alberta, Edmonton, Alberta, Canada

 Post Graduate Education and Training

2000-2001       Postdoctoral Fellow                       

                       University of Maryland School of Medicine, Baltimore, MD

                       Laboratory of Dr. Harry L.T. Mobley – Microbiology and Immunology Department

 Employment History

1995-2000       Graduate Research Assistant

                        University of Alberta

                        Edmonton Alberta, Canada

                        Laboratories of Dr. Glen D. Armstrong and Dr. Diane E. Taylor – Medical Microbiology and Immunology

2001-2002       GenBank Biologist– Computercraft Inc.            

                        National Center for Biotechnology Information

                        National Institutes of Health

                        Bethesda, Maryland

 2002-2005      Staff Scientist/Bioinformatics Analyst

                        The Institute for Genomic Research

                        Microbial Genomics

                        Rockville, Maryland

2005                Collaborative Investigator

                        The Institute for Genomic Research

                        Microbial Genomics

                        Rockville, Maryland

2005-2007       Adjunct Collaborative Investigator

                        The Institute for Genomic Research

                        Microbial Genomics

                        Rockville, Maryland

2005                Senior Research Scientist

                        The University of Texas Southwestern Medical Center at Dallas

                        Department of Microbiology

                        Dallas, Texas

2006-2008       Research Assistant Professor

                        The University of Texas Southwestern Medical Center at Dallas

                        Department of Microbiology

                        Dallas, Texas

2008-2013       Assistant Professor

                        University of Maryland School of Medicine

                        Department of Microbiology and Immunology

                        Institute for Genome Sciences

                        Baltimore, Maryland

2013-2016       Associate Professor

                        University of Maryland School of Medicine

                        Department of Microbiology and Immunology

                        Institute for Genome Sciences

                        Baltimore, Maryland

2015                Awarded Tenure

2016-present  Professor

                        University of Maryland School of Medicine

                        Department of Microbiology and Immunology

                        Institute for Genome Sciences

                        Baltimore, Maryland

Highlighted Publications

Peer-reviewed journal articles (Selected from 133)

  1. D.A. Rasko, M.J. Rosovitz, G.S.A. Myers, E.F. Mongodin, W.F. Fricke, P. Gajer, J. Crabtree, V. Sperandio and J. Ravel. 2008. The pan-genome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates. J Bacteriol. 190(20):6881-93.
  2. D.A. Rasko, C.G. Moreira, D.R. Li, N.C. Reading, J.M. Ritchie, M.K. Waldor, N. Williams, R. Taussig, S. Wei, M. Roth, D.T. Hughes, J.F. Huntley, M.W. Fina, J.R. Falck and V. Sperandio. 2008. Targeting QseC signaling and virulence for antibiotic development. Science. 321(5892):1078-80.
  3. D.A. Rasko and Sperandio. 2009. Novel approaches to bacterial infection therapy by interfering with cell-to-cell signaling. Current Protocols in Microbiology. 2009 Feb;Chapter 17:Unit17.3.
  4. W.F. Fricke, D.A. Rasko and J. Ravel. 2009. The role of genomics in the identification, prediction and prevention of biological threats. PLoS Biology. 7(10):e1000217. Epub 2009 Oct 26.
  5. D.A. Rasko and V. Sperandio. 2010. Anti-virulence strategies to combat bacteria-mediated disease. Nature Reviews Drug Discovery. 9(2):117-28.
  6. R.R. Chaudhuri, M. Sebaihia, J.L. Hobman, M.A. Webber, D.L. Leyton, M.D. Goldberg, A.F. Cunningham, A. Scott-Tucker, P.R. Ferguson, C.M. Thomas, G. Frankel, C.M. Tang, E.G. Dudley, I.S. Roberts, D.A. Rasko, M.J. Pallen, J. Parkhill, J.P. Nataro, N.R. Thomson and I.R. Henderson. 2010. Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042. PLoS One. 20;5(1):e8801.
  7. J.W. Sahl, H. Steinsland, J.C. Redman, S.V. Angiuoli, J.P. Nataro, H. Sommerfelt and D.A. Rasko. 2011. A comparative genomic analysis of diverse clonal types of enterotoxigenic Escherichia coli reveals pathovar-specific conservation. Infection and Immunity. 79(2):950-60.
  8. D.A. Rasko, P. Worsham, T.G. Abshire, S.T. Stanley, J.D. Bannan, M.R. Wilson, R.J. Langham, R.S. Decker, L. Jiang, A.M. Phillipy, S.L. Salzberg, M. Pop, M.N. VanErt, L.J. Kenefic, P.S. Keim, C.M. Fraser-Liggett, J. Ravel. 2011. Microbial forensic applications of comparative genome analysis: Identification of Bacillus anthracis genetic markers in the Amerithrax investigation. Proceedings of the National Academy of Sciences. 108(12):5027-32.
  9. J.W. Sahl, J.K. Johnson, A.D. Harris, A.M. Phillippy, W.W. Hsiao, K.A. Thom, and D.A. Rasko. 2011. Genomic comparison of multi-drug resistant invasive and colonizing Acinetobacter baumannii isolated from diverse human body sites reveals genomic plasticity. BMC Genomics. 4;12:291.
  10. D.A. Rasko, D.R. Webster, J.W. Sahl, A. Bashir, N. Boisen, F. Scheutz, E.E. Paxinos, R.Sebra, C.-S. Chin, D. Iliopoulos, A. Klammer, P. Peluso, L. Lee, A.O. Kislyuk, J. Bullard, A. Kasarskis, S. Wang, J. Eid, D. Rank, J.C. Redman, S.R. Steyert, J. Frimodt-Møller, C. Struve, A.M. Petersen, K.A. Krogfelt, J.P. Nataro, E.E. Schadt, M.K. Waldor. 2011. Origins of the E. coli strain causing a large outbreak of HUS in Germany. New England Journal of Medicine. 365(8):709-17.
  11. J.W. Sahl and D.A. Rasko. 2012. Analysis of the global transcriptional profiles of enterotoxigenic Escherichia coli (ETEC) isolate E24377A. Infection and Immunity. 80(3):1232-42.
  12. T.H. Hazen, G.L. Robinson, A.D. Harris, D.A. Rasko and J.K. Johnson. 2012. Genome sequence of Klebsiella oxytoca 11492-1, a nosocomial isolate possessing a FOX-5 AmpC β-Lactamase. Journal of Bacteriology. 194(11):3028-9. (PMC3370625)
  13. J.W. Sahl, M.N. Matalka and D.A. Rasko. 2012. Phylomark: a tool to find conserved, phylogenetic markers from whole-genome alignments.  Applied and Environmental Microbiology. 78(14):4884-92. (PMC3416384)
  14. S.R. Steyert, J.W. Sahl, C.M. Fraser, L.D. Teel, F. Scheutz and D.A. Rasko. 2012. Comparative genomics and stx phage characterization of LEE-negative Shiga toxin-producing Escherichia coli. Front Cell Infect Microbiol. 2012;2:133. (Open Access article)
  15. E.A. Eloe-Fadrosh and D.A. Rasko. 2013. The human microbiome: from symbiosis to pathogenesis. Annu Rev Med. 2013;64:145-63. (NIHMSID 460863)
  16. C.R. Morris, C.L. Grassel, J.C. Redman, J.W. Sahl, E.M. Barry and D.A. Rasko. 2013. Utilizing transcriptomics to identify mediators of pathogenesis in Shigella flexneri: characterization of intracellular growth regulator, icgR. Infect Immun. 81(9):3068-76.
  17. T.H. Hazen, J.W. Sahl, C.M. Fraser, M.S. Donnenberg, F. Scheutz and D.A Rasko. 2013. Refining the pathovar paradigm via phylogenomics of the attaching and effacing Escherichia coli. Proc Natl Acad Sci USA. 110(31):12810-5.
  18. W.F. Fricke and D.A. Rasko. 2014. Bacterial genome sequencing in the clinic: bioinformatic challenges and solutions. Nat Rev Genet. 15(1):49-55.
  19. T.H. Hazen, L. Zhao, J.W. Sahl, G. Robinson, A.D. Harris, D.A. Rasko and J.K. Johnson. 2014. Characterization of Klebsiella sp. 10982, a colonizer of humans that contains novel antibiotic resistance alleles and exhibits genetic similarities to plant and clinical Klebsiella isolates. Antimicrob Agents Chemother. 58(4):1879-88.
  20. T.H. Hazen, L. Zhao, M.A. Boutin, A. Stancil, G. Robinson, A.D. Harris, D.A. Rasko and J.K. Johnson. 2014. Comparative genomics of an IncA/C multidrug resistance plasmid from Escherichia coli and Klebsiella species isolated from ICU patients: the utility of whole genome sequencing in healthcare settings. Antimicrob Agents Chemother. 58(8):4814-2.
  21. Y. Doi, T.H. Hazen, M. Boitano, Y.C. Tsai, T.A. Clark, J. Korlach and D.A. Rasko. 2014. Whole genome assembly of Klebsiella pneumoniae co-producing NDM-1 and OXA-232 carbapenemases using Single-Molecule, Real-Time Sequencing. Antimicrob Agents Chemother. 58(10):5947-53.
  22. A. von Mentzer, T.R. Connor, L.H. Wieler, T. Semmler, A. Iguchi, N.R. Thomson, D.A. Rasko, E. Joffre, J. Corander, D. Pickard, G. Wiklund, A.M. Svennerholm, A. Sjöling and G. Dougan. 2014. Identification of enterotoxigenic Escherichia coli (ETEC) clades with long-term global distribution. Nat Genet. 46(12):1321-6.
  23. S. Subashchandrabose, T.H. Hazen, A.R. Brumbaugh, S.D. Himpsl, S.N. Smith, R.D. Ernst, D.A. Rasko and H.L.T. Mobley. 2014. Host-specific induction of Escherichia coli fitness genes during human urinary tract infection. Proc Natl Acad Sci U S A. 111(51):18327-32.
  24. J.W. Sahl, C.M. Morris, J. Emberger, C.M. Fraser, J.B. Ochieng, J. Juma, B. Fields, B.F. Breiman, M. Gilmour, J.P. Nataro and D.A. Rasko. 2015. Defining the phylogenomics of Shigella species: A pathway to diagnostics. J Clin Microbiol. 53(3):951-60.
  25. M.S. Donnenberg*, T.H. Hazen, T.H. Fang, S. Panchalingam, M. Antonio, A. Hossain, I. Mandomando, J.B. Ochieng, T. Ramamurthy, B. Tamboura, A. Zaidi, M.M. Levine, K. Kotloff, D.A. Rasko* and J.P. Nataro. 2015. Bacterial factors associated with lethal outcome of Enteropathogenic Escherichia coli infection: genomic case-control studies. PLoS Negl Trop Dis. 2015 May 15;9(5):e000379. (*co-corresponding authors)
  26. J.W. Sahl, J.R. Sistrunk, C.M. Fraser, E. Hine, N. Baby, Y. Begum, Q. Luo, A. Sheikh, F. Qadri, J.M. Fleckenstein and D.A. Rasko. 2015. Examination of the enterotoxigenic Escherichia coli population structure during human infection. MBio. 2015 Jun 9;6(3). pii: e00501-15. doi: 10.1128/mBio.00501-15.
  27. T.H. Hazen, S.C Daugherty, A. Shetty, A.A. Mahurkar, O. White, J.B. Kaper JB and D.A Rasko. 2015. RNA-Seq analysis of isolate- and growth phase-specific differences in the global transcriptomes of enteropathogenic Escherichia coli prototype isolates. Front Microbiol. 2015 Jun 12;6:569. doi: 10.3389/fmicb.2015.00569. eCollection 2015.
  28. T.H. Hazen, J.B. Kaper, J.P. Nataro and D.A. Rasko. 2015. Comparative genomics provides insight into the diversity of the attaching and effacing Escherichia coli virulence plasmids. Infect Immun. 83(10):4103-17.
  29. T.H. Hazen, M.S. Donnenberg*, S. Panchalingam, M. Antonio, A. Hossain, I. Mandomando, J.B. Ochieng, T. Ramamurthy, B. Tamboura, S. Qureshi, F. Quadri, A. Zaidi, K. Kotloff, M.M. Levine, E.M. Barry, J.B. Kaper, D.A. Rasko* and J.P. Nataro. 2016. Genomic diversity of EPEC associated with clinical presentations of differing severity. Nature Microbiology 1, Article number: 15014 doi:10.1038/nmicrobiol.2015.14. (*co-corresponding authors)
  30. D.A. Kania, T.H. Hazen, A. Hossain, J.P. Nataro and D.A. Rasko. 2016. Genome diversity of Shigella boydii. Pathog Dis. 2016 Jun;74(4). pii: ftw027. doi: 10.1093/femspd/ftw027.
  31. T.H. Hazen, S.R. Leonard, K.A. Lampel, D.W. Lacher, A.T. Maurelli and D.A. Rasko. 2016. Investigating the relatedness of enteroinvasive Escherichia coli to other E. coli and Shigella using comparative genomics. Infect Immun. 84(8):2362-71.
  32. L. Wallace, S.C. Daugherty, S. Nagaraj, J.K. Johnson, A.D. Harris and D.A. Rasko. 2016. The use of comparative genomics to characterize the diversity of Acinetobacter baumannii surveillance isolates in a health care institution. Antimicrob Agents Chemother. 60(10):5933-41.