Zeiss LS7 Lightsheet microscope
- Lightsheet microscope for visualization of large 2D and 3D samples.
- Six laser lines: 405, 445, 488, 515, 561, 638 nm.
- Illumination objectives include a 5x/0.1 foc, 10x/0.2 foc.
- Detection objectives for clearing and water-based samples (2.5x, 5x/0.16 NA, 10x/ 0.5 NA, 20x/1 NA).
- Water chamber with temperature control.
- Mesoscale chamber (Translucence Biosystems).
- 2 PCO Edge sCMOS cameras for simultaneous two-color imaging at fast frame rates.
- Zeiss, Arivis, Imaris Bitplane analysis software.
Applications
- Volumetric imaging of Large Sample Imaging (Cleared Tissue), like mouse brains or full organs, with subcellular resolution.
- Long-term, 3D imaging of live model organisms like Drosophila and zebrafish to study embryonic development and morphogenesis without inducing phototoxicity.
- Multiview imaging by combining imaging from multiple angles for high-resolution 3D reconstruction of complex, large samples.
- Imaging of 3D cell culture and organoids by tracking growth, differentiation, and morphology of stem cell-derived organoids or spheroids over days.
- Subcellular dynamics by observing intracellular events, such as trafficking or membrane dynamics, at high speed.