Everything starts with protein. The Protein Engineering & Biophysics (PEB) section engineers and produces purified proteins for investigators, with highly-trained staff offering their expertise and advice for project development. These proteins can be used for any of the other CBT sections, from Target Validation & Screening (TVS) to Structural Biology (SB). 

Capabilities

• Protein expression from a variety of expression systems
• • Bacteria
  • Yeast
  • Baculovirus
  • Mammalian
• Uniform stable isotope labeling (²H,¹³C,¹⁵N,¹⁹F) and selective methyl labeling of proteins for NMR
• Purification of tagged and untagged proteins using HPLC/FPLC techniques
• Characterization of proteins
  • Stability
  • Structure/dynamic properties
  • Size
• Biophysical characterization (SDS-PAGE, FPLC, isothermal calorimetry (ITC)), differential scanning calorimetry (DSC), circular dichroism (CD), fluorescence, UV/Vis, DLS, analytical ultracentrifugation (AUC), kinetic stop-flow/quench-flow, and surface plasmon resonance (SPR) techniques.