Flow Cytometry Shared Service
MISSION:
The University of Maryland Greenebaum Comprehensive Cancer Center Flow Cytometry Shared Service (FCSS) offers equipment and technical expertise to the entire campus, as well as outside clients in conducting research in all areas of basic and applied biomedical sciences.
SERVICES:
- Experimental design, troubleshooting, consultation and grant application
- Cell sorting performed by staff
- Monthly lecture covers basic knowledge, useful tools, and new development. Free and open to all.
- Hands on training on analyzers (LSR II, Canto II, Accuri C6, and FlowSight) Investigators are encouraged to use the analyzers independently.
- Training on analysis software
INSTRUMENTATION:
2 Cytek Aurora Spectral Cytometers
- 3 lasers: 488 nm, 405 nm, 640 nm; 23 colors
- 4 lasers with 355 nm laser, 30 colors
- Ready to use panels provided
- Removes autofluorescence background
- Small particle detection: 100 nm range
Red represents the spillover from the dye in the row to the dye in column
Amnis FlowSight Imaging Cytometer
- 4 lasers: 488 nm, 640 nm, 405 nm, 561nm
- 10 colors
- 20X magnification
- Autosampler
BD Aria II Cell Sorter
- 4 lasers: 488 nm, 633 nm, 405 nm, 552 nm
- 11 colors
- 96-well single cell sorting, 4-way sorting, index sorting
- BSL-2 enhanced sorting inside of bioBUBBLE enclosure
Miltenyi MACSQuant Analyzer 10
- Clinical cytometer
- 3 lasers; 88 nm, 405 nm, 640 nm; 8 colors
- Compliance with FDA 21 CFR Part 11
- Express Modes automate the anaylsis
BD LSR II
- 4 lasers: 488 nm, 640 nm, 405 nm, 561 nm
- 13 colors
BD CANTO II
- 2 lasers: 488 nm, 640 nm
- 6 colors
BD Accuri C6
- 2 lasers: 488 nm, 640 nm
- 4 colors
ANALYSIS SOFTWARE
FCS Express 7 Site License
No charge for facility users.
FlowJo Dongles
PC and Mac dongles can be borrowed at no charge.
