Translational Laboratory

MISSION:

The University of Maryland Greenebaum Comprehensive Cancer Center Translational Laboratory Shared Service (TLSS) offers pre-clinical and clinical
experimental support to basic researchers and physicians in the UMGCCC community. We work in areas across the entire spectrum: cell biology, in vitro, in vivo and human trials.

SERVICES:

In Vitro Assays

• Mycoplasma testing
• Clonogenic Survival Assays
• IC50 generation
• Cell cycle (propidium iodide)
• Viability (trypan blue exclusion)
• Apoptosis
• Potentiation/Synergy
• ROS
• Western Analysis
• Angiogenesis

In Vivo Assays

• IACUC approved umbrella protocol
• Tolerability
• Tumor Growth
• Patient Derived Xenograft Models
• Pharmacokinetics: generation of plasma
• Efficacy (flank models)
• Efficacy (orthotopic models)
• Pharmacodynamic Endpoints
• Imaging of cells with Xenogen System

‌Pharmacodynamic (PD) Endpoints

• in-patient samples, tumor or surrogate tissues, preclinical samples
• Endpoint dependent on target (e.g., ELISA, flow cytometry, Western, unique assay)

CRISPR Services:

• Knock Down and Single Nucleotide Polymorphism

Visit the CRISPR Services Website

RESOURCES:

• Access to >120+ human/murine cell lines
• Luciferase-expressing breast, leukemia, ovarian and prostate cancer cell lines
• IACUC approved umbrella protocol
• Access/Knowledge in Using Xenogen/IVIS Imaging Mice
• Primary Derived Xenograft Models
  • Breast
  • Head and Neck (under development)
  • Leukemia (under development)
  • Ovarian (under development)
  • Pancreatic (under development)