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Translational Laboratory

MISSION:

The University of Maryland Greenebaum Comprehensive Cancer Center Translational Shared Service (TLSS) offers pre-clinical and clinical experimental support to basic researchers and physicians in the UMGCCC community. We work in areas across the entire spectrum: cell biology, in vitro, in vivo and human trials.


SERVICES:

Translational-Lab01In Vitro Assays

  • IC50 generation
  • Cell cycle (propidium iodide)
  • Viability (trypan blue exclusion)
  • Apoptosis
  • Potentiation/Synergy
  • ROS
  • Western Analysis
  • Angiogenesis
  • Mycoplasma testing

Xcelligence

  • Real time proliferation/invasion/migration

Translational-Lab02In Vivo Assays

  • IACUC approved umbrella protocol
  • Tolerability
  • Tumor Growth
  • Pharmacokinetics: generation of plasma
  • Efficacy (flank models)
  • Efficacy (orthotopic models)
  • Pharmacodynamic Endpoints
  • Imaging of cells with Xenogen System

‌Pharmacodynamic (PD) Endpoints

  • in-patient samples, tumor or surrogate tissues,preclinical samples
  • Endpoint dependent on target (e.g., ELISA, flow cytometry, Western, unique assay)

CRISPR Services  (Visit the CRISPR Services Website)

  • Knock Down and Single Nucleotide Polymorphism

RESOURCES:

  • Access to 80+ human cell lines
  • Luciferase-expressing breast, leukemia, ovarian and prostate cancer cell lines
  • IACUC approved umbrella protocol
  • Access/Knowledge in Using Xenogen/IVIS Imaging Mice
  • Primary Xenograft Models
  • Breast
  • Head and Neck (under development)
  • Leukemia (under development)
  • Ovarian (under development)
  • Pancreatic (under development)
Access to IRB approved protocol for tissue acquisition

Translational-Lab04

Clinical Trial support:

We isolate:

  • Plasma
  • Serum
  • Tumor Biopsy
  • Whole Blood (isolation of PBMC, DNA, RNA, protein)
  • Bone Marrow (isolation of marrow cells)
  • Buccal Mucosa

‌‌‌

Translational-Lab05

 

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CONTACT:

Lapidus_Rena

Rena Lapidus, PhD

Director

Tami J. Kingsbury, PhD

Tami J. Kingsbury, PhD

Leader, CRISPR Services

Office: HSF-II, S103C
410-706-1202

Translational Laboratory staff

Brandon Carter-Cooper, MS
Eun Yong Choi, MD
Elizabeth Chang, PhD
Kayla Tighe, BS


LOCATION:

Bressler Research Building
Room 7-010
655 West Baltimore Street
Baltimore, MD 21201


HOURS:

Monday through Friday
7:00am - 5:00pm


PHONE:

Office: 410-706-2171

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