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Translational Genomics Laboratory

MISSION:

The mission of the TranslationalGenomics Laboratory (TGL) is to provide a critical bridge to help accelerate translation from discovery into precision health care, by providing DNA sequencing, genotyping and array-based technologies in both a basic research and clinically-regulatedenvironment.


ABOUT:

The Translational Genomics Laboratory (TGL) is the result of 35 years of core facility evolution, starting with peptide and oligonucleotide synthesis, to the current CAP (College of American Pathologists) accredited and CLIA (Clinical Laboratory Improvement Amendments) compliant laboratory, capable of providing support to clinical and translational genetic/genomics studies (CAP# 8017554; CLIA# 21D2027356). TGL is part of the University of Maryland School of Medicine’s Marlene and Stewart Greenebaum Comprehensive Cancer Center (UMGCC), the Program for Personalized and Genomic Medicine (PPGM), and the Center for Innovative Biomedical Resources (CIBR).

The services offered by the TGL support basic research. The clinical assays offered are similar to those offered to support basic research, except that these assays are validated under CLIA, enabling them to be used in the clinical decision-making processin research protocols and for routine patient care.


SERVICES (Clinical):‌

  • Confirmation of a Research Finding
  • CYP2C19 Genotyping
  • CYP2C19 Sequencing
  • Cytogenomic Microarray
  • Extract and Hold
  • FLT3 ITD and TKD Fragment size Analysis
  • IDH1 R132 and IDH2 R140, R172 Sanger Sequencing
  • Site-specific Familial variant analysis‌
  • Next Gen Sequencing (NGS) Myeloid Malignancy targeted panel

SERVICES (Basic Research):

  • Cytogenomic Arrays
  • Extraction of Nucleic Acid
    • DNA
    • RNA
  • Gene Expression Arrays
    • Global Expression Profiling
    • miRNA Expression Profiling
    • Transcriptome Analysis
  • Genotyping
    • Taqman Assays
  • Next Generation Sequencing  (NGS) Gene Panels
  • Sanger DNA Sequencing

TGL Image 01


‌‌INSTRUMENTATION:

  • Applied Biosystems 3730XL (Sanger sequencing)
  • Affymetrix GeneChip system 3000 7G (chip-based arrays, e.g. CytoScan HD, DMET)
  • Applied Biosystems 7900 RT-PCR system (genotyping by Taqman©-based methods)
  • Applied Biosystems 9700 thermocycler (PCR amplification)
  • Nanodrop single-channel and 8-channel spectrophotometers
  • ThermoFisher QuantStudio 5
  • Ion Torrent Personal Genome Machine (next generation sequencing panels, e.g. Comprehensive Cancer Panel, and Custom AmpliSeq panels)
  • Ion Chef System
  • Ion S5 Sequencer

TGL Image 02a

Quant Studio 5 Amplication Plot

TGL Image 02

Quant Studio 5 Experiment

 

TGL Image 03

ISP Loading/Run Summary


Forms and Documents

CAP Accreditation Certificate

CLIA Certificate of Compliance

Medical Laboratory Permit

QA-506 Clinical Services Test Requisition

QA-514 Clinical Services Test Requisition Extract and Hold Only

QA-36 Clinical Research Project Request

Suggestion Box

Satisfaction Survey


CONTACT:

Ashkan Emadi, MD, PhD

Professor of Medicine
and Pharmacology
Director, Translational
Genomics Laboratory
University of Maryland
School of Medicine
Associate Director for
Clinical Research
Marlene & Stewart Greenebaum Comprehensive Cancer Center
aemadi@umm.edu

Nick Ambulos

Nicholas P. Ambulos, PhD

Director, Genomics Core Facility
nambulos@som.umaryland.edu 

Danielle Sewell, BS

Danielle Sewell, MSRS

Compliance Manager

410-706-3339


LOCATION:

Room 7-037
Bressler Research Building
655 West Baltimore Street
Baltimore, MD 21201


HOURS:

Monday through Friday,
8:00am - 5:00pm


PHONE

Lab: 410-706-3339
Fax: 410-706-0287

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