Translational Genomics Laboratory

MISSION:

The mission of the TranslationalGenomics Laboratory (TGL) is to provide a critical bridge to help accelerate translation from discovery into precision health care, by providing DNA sequencing, genotyping and array-based technologies in both a basic research and clinically-regulatedenvironment.

ABOUT:

The Translational Genomics Laboratory (TGL) is the result of 35 years of core facility evolution, starting with peptide and oligonucleotide synthesis, to the current CAP (College of American Pathologists) accredited and CLIA (Clinical Laboratory Improvement Amendments) compliant laboratory, capable of providing support to clinical and translational genetic/genomics studies (CAP# 8017554; CLIA# 21D2027356). TGL is part of the University of Maryland School of Medicine’s Marlene and Stewart Greenebaum Comprehensive Cancer Center (UMGCC), the Program for Personalized and Genomic Medicine (PPGM), and the Center for Innovative Biomedical Resources (CIBR).

The services offered by the TGL support basic research. The clinical assays offered are similar to those offered to support basic research, except that these assays are validated under CLIA, enabling them to be used in the clinical decision-making processin research protocols and for routine patient care.

SERVICES (Clinical):‌

Confirmation of a Research Finding
CYP2C19 Genotyping
CYP2C19 Sequencing
Cytogenomic Microarray
Extract and Hold
FLT3 ITD and TKD Fragment size Analysis
IDH1 R132 and IDH2 R140, R172 Sanger Sequencing
Site-specific Familial variant analysis‌
Next Gen Sequencing (NGS) Myeloid Malignancy targeted panel

SERVICES (Basic Research):

Cytogenomic Arrays
Extraction of Nucleic Acid
- DNA
- RNA
Gene Expression Arrays
- Global Expression Profiling
- miRNA Expression Profiling
- Transcriptome Analysis
Genotyping
- Taqman Assays
Next Generation Sequencing (NGS) Gene Panels
Sanger DNA Sequencing

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‌‌INSTRUMENTATION:

Applied Biosystems 3730XL (Sanger sequencing)
Affymetrix GeneChip system 3000 7G (chip-based arrays, e.g. CytoScan HD, DMET)
Applied Biosystems 7900 RT-PCR system (genotyping by Taqman©-based methods)
Applied Biosystems 9700 thermocycler (PCR amplification)
Nanodrop single-channel and 8-channel spectrophotometers
ThermoFisher QuantStudio 5
Ion Torrent Personal Genome Machine (next generation sequencing panels, e.g. Comprehensive Cancer Panel, and Custom AmpliSeq panels)
Ion Chef System
Ion S5 Sequencer

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