Microbiology and Immunology
Bressler Research Building, BRB 3-025A
Education and Training
- Pirogov Moscow Medical Institute, Moscow, Russian Federation, BS, Biophysics, 1983
- Institute for Transplantation and Artificial Organs, Moscow, Russian Federation, PhD, 1990
- Uniformed Services University of Health Science, France, Post-Doctoral Fellow, Immunology, 2002
Dr. Toshchakov graduated from the Pirogov Moscow Medical Institute with the B. S. degree in Biophysics and received his Ph.D. degree from the Institute for Transplantation and Artificial Organs, Moscow, Russia for his studies of ischemia-reperfusion injury to isolated organs and cells. His postdoctoral training was in the field of Innate Immunity at the Department of Microbiology and Immunology, Uniformed Services University of Health Sciences, Bethesda with Dr. Stefanie Vogel.
Cell signaling. Toll-like receptors. TIR domains.
Toshchakov V, Jones BW, Perera P-Y, Thomas K, Cody MJ, Zhang S, Williams BRG, Major J, Hamilton TA, Fenton MJ, Vogel SN. TLR4, but not TLR2, mediates IFN-b-induced STAT1 a/b-dependent gene expression in macrophages. Nature Immunol. 2002 April; 3(4); 392-8.
Toshchakov VY, and Vogel SN. Cell-penetrating TIR BB loop decoy peptides: a novel class of TLR signaling inhibitors and a tool to study topology of TIR-TIRinteractions. Exp. Opin. Biol. Ther. 2007; 7(7), 1035-1050. Review.
Toshchakov VY, Szmacinski H, Couture LA, Lakowicz JR, & Vogel SN. Targeting TLR4 Signaling by TLR4 TIR-derived Decoy Peptides: Identification of the TLR4 TIR Dimerization Interface. J Immunol. 2011 Apr; 186(8), 4819-27.
Piao W, Vogel SN, Toshchakov VY. Inhibition of TLR4 Signaling by TRAM-derived Decoy Peptides in Vitro and in Vivo. J. Immunol. 2013 Mar 1; 190(5):2263-72.
Piao W, Shirey KA, Ru LW, Lai W, Szmacinski H, Snyder GA, Sundberg EJ, Lakowicz JR, Vogel SN, Toshchakov VY. A Decoy Peptide that Disrupts TIRAP Recruitment to TLRs Is Protective in a Murine Model of Influenza. Cell Reports 2015 Jun 30; 11(12): 1941-52.
Broadly, our research interests are on the molecular mechanisms that govern the assembly and disassembly of transitory protein complexes in signaling. The focus of current research is on the recruitment of adapter proteins to activated Toll-like receptors (TLR) and consequent initiation of intracellular TLR signaling. Translational significance of our studies is in the development of inhibitors that interfere with formation of oligomeric protein complexes in signaling.