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Sudhakar Kalakonda, PhD

Academic Title:

Research Associate

Primary Appointment:

Pathology

Phone (Primary):

410-706-6310

Education and Training

  • Osmania University, B.Sc, Biology and Chemistry, 1988
  • Osmania University, M.Sc, Genetics, 1990
  • Osmani University, Ph.D, Genetcis, 1998

Biosketch

Dr. Kalakonda received his doctorate degree from Osmania University, Hyderabad, India. Doctoral dissertation was on genetic and biochemical studies in families with essential hypertension. After completing his Ph.D. degree, he worked as a Research Officer in the Department of Genetics, Osmania University, India. During this time, he focused on cytogenetic and molecular aspects of ocular disorders. He joined as Post-Doctoral Research fellow in Dept. of Ophthalmology and Microbiology, University of Maryland.

Dr. Kalakonda joined the faculty of University of Maryland School of Medicine as a Research Associate in 2007. At present he is working in Department of Pathology. His basic interest is to find out molecular aspects of any disease.

Research/Clinical Keywords

Cancer, GRIM-19, Long non coding RNA, Transcribed ultra-conserved region (T-UCR), HIV, Vpr

Highlighted Publications

Kalakonda S, Nallar SC, Jaber S, Keay SK, Rorke E, Munivenkatappa R, Lindner DJ, Fiskum GM, Kalvakolanu DV. Monoallelic loss of tumor suppressor GRIM-19 promotes tumorigenesis in mice. Proc Natl Acad Sci U S A. 2013 Nov 5;110(45):E4213-22 

Kalakonda S, Nallar SC, Lindner DJ, Sun P, Lorenz RR, Lamarre E, Reddy SP, Kalvakolanu DV. GRIM-19 mutations fail to inhibit v-Src-induced oncogenesis. Oncogene. 2014 Jun 12;33(24):3195-204.

Nallar SC, Kalakonda S, Lindner DJ, Lorenz RR, Lamarre E, Weihua X, Kalvakolanu DV. Tumor-derived mutations in the gene associated with retinoid interferon-induced mortality (GRIM-19) disrupt its anti-signal transducer and activator of transcription 3 (STAT3) activity and promote oncogenesis. J Biol Chem. 2013 288(11):7930-41

Kalakonda S, Nallar SC, Gong P, Lindner DJ, Goldblum SE, Reddy SP, Kalvakolanu DV. Tumor suppressive protein gene associated with retinoid-interferon-induced mortality (GRIM)-19 inhibits src-induced oncogenic transformation at multiple levels.Am J Pathol. 2007 Oct;171(4):1352-68.

Kalakonda S, Nallar SC, Lindner DJ, Hu J, Reddy SP, Kalvakolanu DV. Tumor-suppressive activity of the cell death activator GRIM-19 on a constitutively active signal transducer and activator of transcription 3. Cancer Res. 2007 Jul 1;67(13):6212-20.

 

Awards and Affiliations

 

  • Seymour and Vivian Milstein Young Investigator Award, For the year 2006, International Society for Interferon and cytokine Research

 

Lab Techniques and Equipment

Isolation of proteins, SDS-PAGE, Agarose gel electrophoresis, Immunoprecipitation of proteins and western blotting, IHC, Immunofluorescence, Growing and maintaining the Eukaryotic cells, Collection of antibody through hybridoma cells and ascetic fluid from the mice, Transient and stable transfection of different genes into the eukaryotic cells both normal and cancer cells, Isolation of plasmid and restriction enzyme analysis, Cloning and sub-cloning, Isolation RNA from the tissues, making the cDNA and measuring the mRNA by using Real time -PCR analysis, Reporter assay, Animal handling of mice and rat, Colony maintenance, subcutaneous and intraperitoneal injections to mice, xenograft tumor study and chemical induced skin tumorigenesis in mice. Microscopy, Thermocycler, etc