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Rao N. Jaladanki, PhD

Academic Title:

Professor

Primary Appointment:

Surgery

Additional Title:

Research Health Scientist, VA Maryland Health Care System

Location:

HSF-II, 20 Penn Street

Phone (Primary):

410-706-1047

Phone (Secondary):

410-605-7808

Education and Training

Dr. Jaladanki received his doctorate degree in 1992 from Sri Venkateswara University, India. After completing Ph.D. degree, he joined as a Rockefeller Foundation Post-Doctoral Research Fellow in the Department of Physiology, Southern Illinois University, Carbondale, IL and worked in the field of endocrinology during 1993-95. This postdoctoral work formed the foundation for Dr. Jaladanki to pursue the future research on the role of hormones related to Gastrointestinal Physiology. In 1997, he joined the Department of Surgery at University of Maryland School of Medicine and worked in GI research laboratories. His research examined the polyamine mediated GI growth, proliferation, and mucosal restitution signaling pathways. He was promoted to the rank of Professor in the Department of Surgery in 2022.

Research/Clinical Keywords

Gut Mucosal growth and adaptation, cell proliferation, Mucosal Restitution, GI injury and healing, gut barrier function, noncoding RNAs, Posttranscriptional Regulation, cellular polyamines

Highlighted Publications

Publications (Selected from 125 articles published as Jaladanki N. Rao)

 

  1. Wang SR, Rathor N, Kwon MS, Xiao L, Chung HK, Turner DJ, Wang JY, and Rao JN. miR-195 Regulates Intestinal Epithelial Restitution after Wounding by altering Actin-Related Protein-2 Translation.  Am J Physiol Cell Physiol,  322:C712-C722, 2022.
  2. Xiao L, Ma X-X, Luo J, Chung HK, Yu TX, Kwon MS, Rao JN, Kozar R, Gorospe M, and Wang JY. Circular RNA circHIPK3 promotes homeostasis of the intestinal epithelium by reducing miR-29b function. Gastroenterology,  161:1303-1317, 2021.
  3. Rathor N, Chung HK, Wang SR, Song J-L, Wang J-Y, and Rao JN. TRPC1-mediated Ca2+ signaling enhances intestinal epithelial restitution by increasing a4 association with PP2Ac after wounding.    Physiol Rep, 9: e14864, 2021.
  4. Rao JN, Xiao L, and Wang J-Y. Polyamines in Gut Epithelial Renewal and Barrier Function.  Physiology,  35: 328-337, 2020.
  5. Liu L, Xiao L, Chung HK, Kwon MS, Li X-X, Wu N, Rao JN, and Wang JY. RNA-binding protein HuR regulates Rac1 nucleocytoplasmic shuttling through nucleophosmin in the intestinal epithelium.  Cellular and Molecular Gastroenterology and Hepatology, 8:475-486, 2019.
  6. Jiang LP, Wang SR, Chung HK, Buddula S, Wang J-Y, and Rao JN. miR-222 represses expression of zipcode binding protein-1 and phospholipase C-γ1 in intestinal epithelial cells.  Am J Physiol Cell Physiol,  316:C415-C423, 2019.
  7. Chung HK, Wang SR, Xiao L, Rathor N, Turner DJ, Yang P, Gorospe M, Rao JN and Wang J-Y. a4 coordinates small intestinal epithelium homeostasis by regulating stability of HuR.  Mol Cell Biol,  38:e00631-17, 2018.
  8. Rathor N, Chung HK, Wang SR, Qian M, Turner DJ, Wang J-Y, and Rao JN. b-PIX plays important role in regulation of intestinal epithelial restitution by interacting with GIT1 and Rac1 after wounding.  Am J Physiol Gastrointest and Liver Physiol, 314:G-399-G407, 2018.
  9. Wang PY, Wang SR, Xiao L, Chen J, Wang J-Y and Rao JN. c-Jun Enhances Intestinal Epithelial Restitution after Wounding by Increasing Phospholipase C-γ1 Transcription.  Am J Physiol Cell Physiol, 312: C367-C375, 2017.
  10. Chung HK, Rathor N, Wang SR, Wang J-Y, and Rao JN. RhoA enhances store-operated Ca2+ entry and intestinal epithelial restitution by interacting with TRPC1 after wounding.  Am J Physiol Gastrointest and Liver Physiol, 309:  G759-G767, 2015.

Additional Publication Citations

https://www.ncbi.nlm.nih.gov/sites/myncbi/1FoEh-V_G1B5K/bibliography/41493572/public/?sort=date&direction=descending

Research Interests

My research is intended to elucidate the mechanistic roles of Ca2+-permeable channels in the regulation of intestinal epithelial restitution after wounding and their regulation by cellular polyamines. Continuing along these lines, our group has performed cell imaging/MS-2 system to localize specific mRNA subcellular distribution and mRNA traffic into/out from processing-body/stress granules during restitution and has defined the involvement of RNA-binding proteins and microRNAs in the regulation of acute mucosal injury and repair. Our team also has developed innovative experimental approaches that utilize the difference between multiple methods and stimulus modalities to infer underlying mechanisms of gut mucosal injury/repair and barrier dysfunction. The data generated also strengthens our long-term goal to develop therapeutic approaches for GI mucosal injury-related diseases and to maintain epithelial integrity under various critical surgical conditions.  

Clinical Specialty Details

N/A

Awards and Affiliations

Dr. Jaladanki has also been actively involved in basic surgical research teaching in Cell Biology Group over the past two decades. He instructs young research fellows, surgical residents, medical students, and college students at the lab and serves as a mentor for college students during the period of summer research. He is also involved with various Baltimore VAMC and UMB institutional committees. Dr. Jaladanki has also been an active member in scientific societies, including American Gastroenterology Association and American Physiological Society.

Grants and Contracts

Department of Veterans Affairs MERIT Award and National Institutes of Health

Lab Techniques and Equipment

Lab Techniques and Equipment

• Gut mucosal injury models in vitro and in vivo
• small GTP binding proteins, GPCRs during wound healing
• Measurements of paracellular permeability and barrier functions
• microRNAs regulation on gut mucosal integrity
• RNA-pull down assays

• Polysome profile analysis
• Transient (adenoviral system) and stable gene transfection
• Promoter deletion, exchange, and point mutations and reporter gene assays
• Fluorescence measurement of intracellular Ca2+ concentration

• miRNA profiles and RNA Binding Proteins