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Leonardo N. DeTolla, MD

Academic Title:

Research Associate

Primary Appointment:

Microbiology and Immunology

Additional Title:

Research Associate, Department of Microbiology and Immunology


10 South Pine Street

Phone (Primary):

(410) 706-3909


(410) 706-8538

Education and Training

Towson University, BA, Biology, 2003

Poznan University of Medical Sciences, MD, Medicine, 2014



Medical Training in Pediatrics
Union Hospital, Elkton, MD

Medical Training in Gynecology and Obstetrics
University of Maryland Medical Center, Baltimore, MD


Medical Training in General Surgery
Johns Hopkins University Hospital, Baltimore, MD

Medical Training in Emergency Medicine
Union Hospital, Elkton, MD


Medical Training in Internal Medicine
Dr. Hsu’s Primary Care Clinic, Elkton, MD


Medical Training in the Outpatient Clinic-Family Medicine

In 2015 he was appointed as a Visiting Scientist/ Research Associate in the Department of Microbiology and Immunology at The University of Maryland School of Medicine.  He has supported laboratory studies in diagnostics using direct culture techniques for bacteria, PCR and cell culture.  He is also familiar with the use of ELISA and similar assays to detect antibodies to pathogens in a range of animal species. Most recently he has developed expertise in the operation of a germ free facility to support the use of mouse models in various microbiome studies.

Research/Clinical Keywords

Microbiome, GermFree mice, Gut-Brain interactions

Highlighted Publications

One Health at The University of Maryland, Baltimore

Contel, CM, Hungerfold, LL, DeTolla, LN and Coksaygan, T.,  University of Maryland School of Medicine, Comparative Medicine Program and Departments of Epidemiology, Public Health and Pathology

Presented at the National Meeting of the American College of Laboratory Animal Medicine, 2014

Professional Activity

Flow Cytometry experiments with Faculty from the Department of Microbiology and Immunology Fall, 2019

Lab Techniques and Equipment

1. Tissue culture

2. Operate and maintain laboratory equipment

3. Support production of monoclonal antibodies

4. PCR, ELISA and other immunoassays

5. Inventory media, supplies and request ordering as needed

6. Project support as neeeded