Animal Model Phenotyping Core
Director: Liqing "Justin" Yu, MD, PhD
- Univesity of Maryland
- Johns Hopkins
Metabolic phenotyping of animal models under different nutritional, environmental, pathophysiological, and pharmacological conditions has become an integral part of most NIH-funded research in basic and translational sciences. The use of animal models is expected to increase significantly and to cover diverse animal species with the advent of novel and simple gene-editing technologies. To meet these needs, we have designed a new Animal Model Phenotyping (AMP) Core for this renewal cycle of our NORC. The goal of the AMP Core is to provide a variety of high-quality animal phenotyping and metabolic analysis services essential for Nutrition and Obesity research.
The AMP Core is a new Core at Mid-Atlantic NORC. The Core will offer the following services:
1) Energy balance studies using TSE PhenoMaster Metabolic Cage system that measures energy expenditure and respiratory exchange ratio (by Indirect Calorimetry), water/food intake, and physical activity in awake mice;
2)Continuous recording of many physiological parameters, including changes in core body and tissue temperatures using the state-of the art Stellar Telemetry system;
3) Body composition analysis of mice using EchoMRI;
4) A series of in vivo analysis of nutrient metabolism, including hyperinsulinemic-euglycemic clamp studies, tissue glucose uptake, glucose tolerance test, insulin tolerance test, tissue fatty acid synthesis/uptake/oxidation; and (5) Assays of blood glucose, lipids, hormones, and adipokines.