Protein Engineering & Biophysics
The goal of the Protein Engineering and Biophysics (PEB) section is to produce purified proteins for investigators, for use other CBT sections (i.e. Structural Biology (SB) for NMR and crystallization and Target Validation & Screening (TVS) for use in in vitro assays), and for internal studies by the PPB section. The PPB has assembled the expertise to provide a wide range of methods for studying the biophysical properties of proteins including protein-ligand and protein-protein interactions.
- Protein expression from a variety of expression systems
- Uniform stable isotope labeling (2H,13C,15N,19F) and selective methyl labeling of proteins for NMR
- Purification of tagged and untagged proteins using HPLC/FPLC techniques
- Characterization of proteins
- Structure/dynamic properties
- Covalent modification (phosphorylation, ubiquitination, methylation, glycosylation, etc.)
- Characterization of unknown proteins (SDS-PAGE, FPLC, Mass Spec (MS), isothermal calorimetry (ITC), ), nuclear magnetic resonance (NMR), differential scanning calorimetry (DSC), circular dichroism (CD), fluorescence, UV/Vis, DLS, analytical ultracentrifugation (AUC), kinetic stop-flow/quench-flow, and surface plasmon resonance (SPR) techniques.
- Measuring enzyme kinetics, binding kinetics, and thermodynamic properties of a enzyme/proteins