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Frank L. Margolis, PhD

Academic Title:

Professor Emeritus

Primary Appointment:

Anatomy and Neurobiology

Secondary Appointment(s):

Psychiatry

Additional Title:

Director, MS Program in Molecular Medicine

Location:

HSFII, S203-D

Phone (Primary):

(410) 706-8913

Phone (Secondary):

(410) 706-8914

Fax:

(410) 706-2512

Education and Training

  • Antioch College, Yellow Springs, OH, 1954-1959, B.S. Chemistry
  • Columbia U., New York City, NY, 1959-1964, Ph.D. Biochemistry
  • Columbia U., New York City, NY, 1964-1965, Postdoctorate, Biochem.
  • Lab de Physiologie Comp, Universite de Paris, 1965-1966, Postdoctorate, Fetal endocrinology

Biosketch

The olfactory marker protein (OMP) was discovered by my laboratory many years ago. We showed that expression of this protein is virtually exclusively restricted to mature olfactory sensory neurons that are responsible for the sense of smell. The primary amino acid sequence of OMP was determined to be 50% identical in all vertebrate species, absent from invertebrates, and devoid of any sequence motifs that could give a hint as to function. To address function we generated knock-out animals and characterized the phenotype of these mice to obtain insight to function. OMP clearly plays a role in olfaction as demonstrated by behavioral analyses illustrating that its absence results in the need for a 50-100 fold higher concentration of odorant needed for behavioral response. In addition, analyses by voltage sensitive dyes illustrated that the OMP-KO mice were compromised in their response to odorants. Proof of function was further demonstrated by rescue of the OMP-KO phenotype by infection of the olfactory neurons with an OMP expressing adenovirus. To address mechanism, studies in my lab have continued in several directions:1-determination of the 3-D solution structure of OMP, 2-analysis of the role of OMP in Ca kinetics following odor stimulation and 3-identification of a protein binding partner. Our Ca imaging studies of the OMP-KO mice implicated Na/Ca-exchanger (NCX) activity was compromised in OMP-KO mice.

The 3-D structure of OMP has a beta-clam structure with an omega loop predictive of protein–protein interaction. Search for a protein partner led to the discovery of the interaction of OMP with the Bex1 and Bex2 proteins. Unlike OMP, these partner proteins have several active motifs including the ability to bind Ca/CaM. These studies have led us to the hypothesis that OMP is involved in regulating Ca kinetics in mature olfactory neurons. That observation, coupled with the olfactory behavioral deficit observed in the Bex1-KO mice, supported the hypothesis that OMP and Bex1 interacted to form a complex that regulated the participation of Ca/CaM in olfactory transduction. Further we proposed that Ca/CaM is a regulator of NCX activity and that the interaction of OMP/Bex/CaM is a regulatory triad that influences CaM availability to various steps in the olfactory transduction cascade. OMP does indeed bind to several Ca/CaM binding motifs in several proteins involved in olfactory transduction consistent with the hypothesis that OMP is a modulator of Ca/CaM activity or of other proteins that influence Ca availability in the cell. Thus, my expertise in olfactory function, protein-protein interaction, and role of regulators of NCX activity provide compelling reasons for my participation as a mentor on this grant proposal.

Research/Clinical Keywords

Chemosensory, olfaction, gene regulation,

Highlighted Publications

Koo JH, Smiley M, Lovering R and Margolis FL: Bex1 knock out mice show altered skeletal muscle regeneration Biochem Biophys Res Comm 363 pp 405-410 (2007)

Bettini E. and Margolis FL. Expression of an intron containing beta-tubulin mRNA in catfish olfactory epithelium J. Molec Histol. 38:571-579(2007).

Pyrski M, Koo JH, Polumuri S, Ruknudin A, Margolis JW, Schulze DH and Margolis FL Sodium-calcium exchanger expression in the mouse and rat olfactory systems J Comp Neurol 501:944-958 (2007).

Koo JH and Margolis FL: Bex1 knock out mice show altered skeletal muscle regeneration Biochem Biophys Res Comm 363 pp 405-410 (2007)

Reisert J, Yau KW Margolis FL Olfactory marker protein modulates the cAMP kinetics of the odour-induced response in cilia of mouse olfactory receptor neurons J.Physiol 585: 731-740 (2007).

Additional Publication Citations

Youngentob, S. and Margolis F.L.: OMP gene causes an elevation in behavioral threshold sensitivity. Neuroreport 10: 15-19 (1999).

Baumeister H., Gronostajski, R.M., Lyons G.E., Margolis F.L.: Identification of NFI-binding sites and cloning of NFI-cDNAs suggest a regulatory role for NFI transcription factors in olfactory neuron gene expression. Brain Res. Mol. Brain Res72: 65-79 (1999).

Baker H., Cummings D.M., Munger S.D., Margolis J.W., Franzen L., Reed R.R., and Margolis F.L.: Targeted deletion of a cyclic nucleotide-gated channel subunit (OCNC1): biochemical and morphological consequences in adult mice.  J. Neurosci.19: 9313-9321 (1999).

Cummings, D.M., Emge D.K., Small, S.L. and Margolis, F.L.: The pattern of olfactory bulb re-innervation returns following recovery from reversible peripheral deafferentation. J. Comp. Neurol421:362-73 (2000).

Ivic L., Pyrski M.M., Margolis J.W., Richards L.J., Firestein, S., Margolis, F.L.: Adenoviral vector-mediated rescue of the OMP-null phenotype in vivo.  Nature Neurosci11:1113-1120. (2000).

Behrens, M., Venkatraman, G., Gronostajski, R.M., Reed, R.R. and Margolis F.L.:  NFI in the development of the olfactory neuroepithelium and the regulation of the olfactory marker protein (OMP) expression.  Eur. J. Neuroscience12:1372-84 (2000).

Baldisseri, D.M., Margolis, J.W., Omotosho P.A. and Margolis F.L.:  Sequence-specific NMR resonance assignments of the backbone atoms for the olfactory marker protein, OMP. J. Biomolecular NMR 17:353-354 (2000).

Pyrski M, Xu Z, Walters E, Gilbert DJ, Jenkins NJ, Copeland NJ, and Margolis FL: The OMP-lacZ transgene mimics the unusual expression pattern of OR-Z6, a new odorant receptor gene on mouse chromosome 6: implication for locus-dependent gene-expression. J. Neurosci.: 21: 4637-4648 (2001).

D.H. Schulze, A. Ruknudin, J.W. Margolis, S.K. Polumuri, F.L. Margolis, Sodium Calcium Exchangers in Olfactory Tissue. "Cellular and molecular physiology of Sodium-Calcium exchange," Banff, October, (2001).

Baldisseri DM, Margolis JW, Weber DJ, Koo JH, Margolis FL. Olfactory marker protein (OMP) exhibits a beta-clam fold in solution.  Implications for target peptide interaction and olfactory signal transduction. J Molec Biol 319:823-837 (2002).

Perretto, P Cummings, DM, Modena C,  Behrens M,  Venkatraman G, Fasolo A, Margolis FL: BMP mRNA and protein expression in the developing mouse olfactory system J. Comp. Neurol451:267-278 (2002).

Schulze DH, Pyrski M, Ruknudin A, Margolis JW,  Polumuri SK, Margolis FL. Sodium-calcium exchangers in olfactory tissue.  in Lytton J, Schnetkamp PPM, Hryshko LV and Blaustein MP (Editors) "Cellular and molecular physiology of sodium-calcium exchange" Ann. NY Acad. Sci976, 67-72 (2002).

Research Interests

The vertebrate olfactory system is an extremely plastic neurobiological substrate that provides a platform from which one can address questions relating to sensory transduction, neuronal ontogeny, plasticity, regeneration and transynaptic regulation of gene expression. One of the major goals of my laboratory is to identify and characterize the molecular mechanisms responsible for regulating olfactory neuron gene expression, and to learn how they contribute to the formation, organization and function of this critical chemosensory system.

We use a multidisciplinary strategy emphasizing biochemical and molecular biological approaches. Promoter motifs involved in regulating olfactory neuron gene expression have been characterized in vitro by gel-shift and footprinting analyses and in vivo by the generation of transgenic mice using truncated and mutated promoter constructs. This latter approach has been used to selectively engineer heterologous gene expression in olfactory neurons.

Primary olfactory neurons are constantly being replaced from a population of precursor cells in the nasal neuroepithelium. These new neurons reinnervate the olfactory bulb. Two questions that arise as a result of this observations are 1) Is reinnervation topographically correct? 2) What are the mechanisms that participate in this process? To address the former we have used transgenic mice expressing the lacZ gene in a subset of cells to demonstrate, for the first time, that reinnervation of the bulb after deafferentation is topographically correct. To address the second question we have begun to characterize the patterns of expression during development and in response to lesion of the BMP family of cytokines and their modulators (noggin, chordin, follistatin and tolloid). These molecules are differentially expressed in various neuronal populations in the olfactory system in spatial and temporal patterns that implies that they participate in development and reorganization of this system. these processes. This is a new and active direction for the laboratory.

An ongoing question in the lab is the function of OMP, a novel phylogenetically conserved, cytoplasmic protein. To this end we have created "knock-out" mice. These mice are superficially normal, but manifest reduced olfactory neural activity. This is evident both electrophysiologically and behaviorally. These observations imply that OMP is a novel regulator of olfactory signal detection/transduction. To address this we are utilizing electrophysiological, behavioral and biochemical approaches. We have "rescued" the electrophysiological phenotype by transfection of olfactory neurons with OMP expressing adenovirus. Attempts to do the same for the behavioral deficit are underway. Biochemical approaches to this problem include the use of 1)solution NMR to determine the 3-D structure of OMP, 2)biochemical studies of protein-protein interaction and 3) phage display to identify and characterize the molecular components responsible for the phenotype observed.

Research Support: Current and past three years

5R01 DC03112-10               F.L. Margolis (PI)                                    Current  04/01/97-08/31/12                 

NIH/NIDCD        Title:OMP:Role in olfactory signal detection and transduction

The major goals of this project are to identify and characterize the biochemical mechanism by which OMP functions, to identify partner molecules and to characterize and rescue the phenotype of the OMP-null mutant.

 

ABMRF                                                            F.L. Margolis (PI)                                                                                             Current 6/01/06-12/31/08 Completed                 

Title: Neuronal loss and replacement after ethanol and abstinence.

The major goal of this grant is to determine the effect of ethanol administration on olfactory neuron loss and recovery in control and transgenic mice.

 

5T32 DC00054                              F. L. Margolis (PI)                              07/01/97 – 06/30/07  Completed                      

NIH- NIDCD Title:Training Program in Chemosensory Neuroscience

The major goals of this training program are to train postdoctoral fellows in chemosensory neurosciences.

 

5R21 NS045841-03              F.L. Margolis (PI)                                         01/31/04 –01/31/07  Completed

NIH-NINDS

Title: Molecular regulation of human callosal development. Our goal is to determine how ACC occurs in humans and what factors are common among ACC and other commissural defects occur. 

Awards and Affiliations

Other Experience and Professional Memberships

1970-date       American Association for the Advancement of Science

1973-date       American Society for Biochemistry and Molecular Biology

1980-2008      American Society for Pharmacology and Experimental Therapeutics

1970-2007      American Society for Neurochemistry

1975-2007      International Society for Neurochemistry

1975-date       Society for Neuroscience

1964               Phi Lambda Upsilon

1970-date       Sigma Xi

1978-date       Association for Chemoreception Sciences

 2005-2006     International Society for Developmental Neuroscience

 2005-2008     Research Society for Alcoholism

Listed in "American Men and Women of Science", "Who's Who in the East""Who's Who in Frontier Science and Technology"

1990-to date    Member: N.I.H. Site Visit Teams, N.S.F. Graduate Fellowship Panel, Ad Hoc Member, Sensory     Disorders and Language Study Section, IFCN4, NIDCD, HFSP, Expert Panel, Smell, Taste, Touch and Chemosensory Disorders, NIDCD, Grant reviewer for Israel Binational Research Found., Swiss National Science Found., US National Science Foundation, Internal Advisory Committee, Howard University SNRP NINDS (1999-2004 ), External Advisory Committee, Howard University SNRP NINDS (2004-2006), Scientific consultant Drinker, Biddle and Reath LLP (2004-2006), Scientific Consultant Telethon  Foundation, Italy  (2007), Scientific Consultant AgraQuest Inc. (2008-date),  NIIH Special Emphasis Panel/Scientific Review Group ZRG1 F01-E (2009), NIH Fellowship review Panel “Brain disorders and related neuroscience”, BPNS ZRG1 F01W (2009), Member external review panel, Univ. Utah Graduate Program in Neuroscience (2009).

Honors

1959 – 1966 Graduate and Postdoctoral Fellowships from NIH and NSF

1985    S. Freeman Memorial Award for "Innovative Research in the Chemoreception Sciences"

1986    Philips Memorial Lecturer in Biology, Haverford College, Haverford, PA

1992    Medalist - LVMH Award "Science pour l'art".

1993    Recipient of the "Frank Allison Linville's R.H. Wright Award in Olfactory Research"

1994    Panel Member, Research Opportunities in Biology and Medicine, ONR-NRC

1995    Panel Recipient of the Olfactory Research Fund's "Scientific Sense of Smell Award

2004    Visiting Professor: Kansas State University, NSF-ADVANCE Program, Manhattan KS March 2004 

Grants and Contracts

5R01 DC03112-10               F.L. Margolis (PI)                                                                                                            04/01/97-08/31/12                  

NIH/NIDCD        Title:OMP:Role in olfactory signal detection and transduction

The major goals of this project are to identify and characterize the biochemical mechanism by which OMP functions, to identify partner molecules and to characterize and rescue the phenotype of the OMP-null mutant.

 

ABMRF                                                            F.L. Margolis (PI)                                                                                                                                             6/01/06-12/31/08                  

Title: Neuronal loss and replacement after ethanol and abstinence.

The major goal of this grant is to determine the effect of ethanol administration on olfactory neuron loss and recovery in control and transgenic mice.

 

5T32 DC00054                              F. L. Margolis (PI)                                                                            07/01/97 – 06/30/07                     

NIH- NIDCD Title:Training Program in Chemosensory Neuroscience

The major goals of this training program are to train postdoctoral fellows in chemosensory neurosciences.

 

5R21 NS045841-03              F.L. Margolis (PI)                                                                                    01/31/04 –01/31/07    

NIH-NINDS

Title: Molecular regulation of human callosal development. Our goal is to determine how ACC occurs in humans and what factors are common among ACC and other commissural defects occur.  

Professional Activity

Administrative Service Institutional Service

Roche Institute of Molecular Biology

1975-1995Ph.D. Thesis Oversight Committees 1977-1978,1994 Staff Senate President

1976-1985 1992-1993 1978-1990 1976-1980 1980-1988 1985-1994 1984-1988 1988-1989 1988-1994

Departmental Seminar Committee Departmental Seminar Committee

Chair, Animal Care Committee Safety Committee Library Committee Institute and Departmental Search Committees Neuron Journal Club Organizer

Acting Chairman, Department of Neurosciences Supervisor, Departmental DNA sequencing and oligonucleotide synthesis

facility

1978-1991 1982-1985 1986-1991 1982-1985

Institutional Animal Care and Use Committee Chairman, Committee on Animal Research Protocol Review Member, Committee on Animal Research Protocol Review Mentor, High School Science Teacher Training Program

Hoffmann-La Roche Inc.

City University of New York, NY

1979Co-organizer Graduate Neurochemistry Course 1978-1990Second Level Examining Committee and Ph.D. Thesis Committees

University of Maryland School of Medicine

2000 2001 2003 1996-2005

1996-2002

1996-2004 1996-present 1997 1997 1997-present 1998

1998

1999-2010 2000-2005

2000-2003 2001-2009 2001-2003

Intramural Grant Review Committee (REEF) GRA Allocation Advisory Committee REEF Instrumentation Award Committee Faculty Search Committee, Dept. of Anatomy & Neurobiology

(Chair, 1996, 2000, 2005) Space and Resources Committee, Dept. of Anatomy & Neurobiology

(Chair, 2000-2002) Graduate Committee, Dept. of Anatomy & Neurobiology Chairman's Advisory Committee, Dept. of Anatomy & Neurobiology Alternate, Faculty Council Seminar Committee, Dept. of Anatomy & Neurobiology Training Committee, Program in Neuroscience Representative, Faculty Council Dept. Biochemistry and Molecular Biology Advisory Committee on Molecular Structure

Transgenic Advisory Committee Dept. Biochem/Molec.Biol.NMR protein structure facility advisory

Committee Executive Committee, Faculty Representative Curriculum Committee, Program in Neuroscience UMB SOM Appointments, Promotions and Tenure Committee

Frank L. Margolis

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32001-present

2001-2002 2002 2002

2002 2002-2003 2002-2003

2002-2013 2003 2003-2010 2004 2004-2005 2005, 2007 2005-2006 2006-2009 2006-2008 2006-2010 2007- 2009 2009

2010-2014 2012 2012-present 2012-present

Local and National Service

1970-present

1970-present 1970-present

1975-1981 1977

1978 1979-1981

External or Ad Hoc Reviewer for Grant Proposals: NIH, NSF, VA, Research Corp., U.S. Army, Hoffmann-La Roche-CMDNJ Foundation, Alzheimer’s Disease Foundation Tobacco Research Council, NIH Special Study sections(2x/year)

Advisory Consultant: NIH Site Visit Teams, Ad hoc reviewer for various NIH Study Sections.(2x/year)

Reviewer for manuscripts: Brain Research; J. Neurochem.; Arch. Biochem. Biophys., Proc. Natl. Acad. Sci., J. Biol. Chem., Anal. Biochem, Science,

Nature, J. Neurosci., J. Comp. Neurol. etc. (3-5x/year). Neurochemical Research Editorial Advisory Board

Member NSF Select Panel: “Assessment of Needs of the Chemosensory Research Community in the United States” Association for Chemoreception Sciences (AChemS): Organizing Committee

Member Grants Review Panel: NSF Sensory Physiology and Perception Program

1984-1994

1982-1994 1986

American Society for Neurochemistry, Fundraising Committee on Travel for Young Investigators Chemical Senses Editorial Board Sigma Xi: Treasurer

Frank L. Margolis

Promotion and Tenure Committee, Dept. of Anatomy & Neurobiology (Chair, 2004, 2005) Biopolymer Facility Oversight Committee Search for Chair, Dept. Physiology ad hoc member

Co-organizer, Symposium “Frontiers in Sensory Biology: Translation to Therapy”, Program in Neuroscience Discussion leader, Tri-Campus PIN meeting Chair, Search for Director of Transgenic Facility

Space Allocation Advisory Committee HSF-2, Dept. of Anatomy & Neurobiology

Qualifying Examination Committees, Program in Neuroscience Chair, Session on Transgenics, SOM-Research Retreat Chair, Faculty Advisory Committee Transgenic Core Facility Dept. of Psychiatry Faculty Search ad hoc member

Co-chair, Faculty Search Committee, Dept. of Anatomy & Neurobiology RAAC Core Facility Review Committee Member Search Comm. for Chair of Dept. Biochem. Molec. Biol. GPILS Curriculum Development Committee

GPILS Training Grant Committee Director of Graduate Studies, Program in Neuroscience Dean’s Scientific Advisory Council

Internal Advisory Board Metabolic and developmental aspects of mental retardation, Mary C. McKenna, Ph.D., P.I. Chair, Departmental mentoring committee for pre-tenured faculty member Member Committee on Research Misconduct

Member UMB IACUC Director Graduate Studies Molecular Medicine MS Program

4

American Society for Neurochemistry: Program Committee International Society for Neurochemistry: Committee on Nominations Sigma Xi Nominating Committee American Society for Neurochemistry Councilor

1979, 1981 1981-1982 1981 1979-1983 1983, 1990-1991Sigma Xi: Admissions Committee

4

Frank L. Margolis

1990AChemS Awards Committee 1990, 1992, 1996, 2013 Member: NSF Graduate Fellowship Panel 1991National Science Scholars Program, N.J. State Nominating Committee 1992, 2009-2012 AChemS Program Committee

5

1993

1993 1993 1993 1994

1994

1994-1996 1999-2004 2003-2004 2004-2006 2004-2006 2005-2006 2007 2008-2013 2009

2009

2009 2009 2010 2012 2013 2014

Member: Smell, Taste and Touch and Chemosensory Disorders Expert Panel To Update The National Strategic Research Plan, NIH/NIDCD Special Member: Sensory Disorders and Language Study Section, NIH/NIDCD Neurobiology Editorial Board

Member: External Advisory Committee, Smell and Taste Center, Univ. of Pa. Member: External Advisory Committee, Monell Center for the Chemical Senses Member: Panel on ONR Research Opportunities in Biology and Medicine/Naval Studies Board, National Research Council

Governing Committee for the R.H. Wright Award in Olfactory Research Internal Advisory Committee, Howard University SNRP NINDS External Advisory Committee Dept. Biochem SUNY Buffalo PPG External Advisory Committee, Howard University SNRP NINDS

Scientific consultant Drinker, Biddle and Reath LLP Eligibility Consultant: NSF Graduate Research Fellowship Program

Scientific Consultant Telethon Foundation, Italy Scientific Consultant AgraQuest Inc.

External consultant NIH SEP SRG ZRG1 F01-E

Fellowship review Panel Brain Disorders and related Neuroscience, BPNS ZRG1 F01W

Internal reviewer UM Dental School T32 Training Grant External reviewer Univ. of Utah Program in Neuroscience NIH CDRC Fellowship Review Panel Reviewer AIBS Military Medical Research and Development Scientific consultant Patton Boggs LLC

Scientific consultant US Army Corps of Engineers, Engineer Researchand Development Center (ERDC)

Teaching Service Teaching Medical Student Teaching

1998-2000Medical Neuroscience Group leader “Neuronal Stem Cells” UMB School of Medicine

2010-presentStructure and Development, “Epithelium”, UMB School of Medicine

Post-Graduate Teaching

1979Co-organizer and instructor: Graduate Neurochemistry Course City University of New York, 25 students, 2 contact hours per week

1999Lecturer: Developmental Neurobiology Course UMB School of Medicine, 15 students, 3-5 contact hours/year

2001-present Discussion group leader: CIPP 907 Research Ethics, 12 students 2-4 contact hours/year

2004-2007Co-course master: CIPP 907 Research Ethics, 40 students,15-20 contact hours/year

2001Molecular Neurobiology Section Head, Intro to Neurosciences NACS 641, UMB School of Medicine, 15 students, 5-10 contact hours/year

2002Group leader, “Intro to Grantspersonship” 30 students, 2-3 contact hours/year

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2002-2013 2006-2009 2014

1970-1975 1977-1981

1996-2001 1996-2004

2001-2005 2003-2006

Angelica Keller: “Studies with a Protein Purified from Rat Olfactory Bulbs That is Specific to the Olfactory Chemoreceptor Neuron”, Graduate Department of Biochemistry, City University of New York Sarah Rochel: “Biochemical Events Associated with Neuronal Regeneration and Synaptic Function in the Olfactory Pathway”, Graduate Department of Biochemistry, City University of New York

Martina Pyrski: “Characterization of the OR-Z6 gene” – Department of Anatomy and Neurobiology, UMB School of Medicine, Baltimore MD

Walter Kostich: “Characterization of primate corticotrophin releasing factor receptors”, Department of Anatomy and Neurobiology, UMB School of Medicine, Baltimore MD Yiling Nie – “Taste transduction.” Department of Anatomy and Neurobiology, UMB School of Medicine, Baltimore MD (co-Mentor with S. Munger).

Hyun Hee Kim- Transynaptic effects in piriform cortex of olfactory nerve lesion” Department of Anatomy and Neurobiology, UMB School of Medicine, Baltimore MD

Frank L. Margolis

GPLS-737 Proseminar, UMB School of Medicine, 10 students, 40-50 contact hours/year

Organizer “Alternate Careers in Biological Science” 25 students, 5-10 contact hours/year Organizer, Brown Bag Research Rounds Mol. Med. MS Program, 10 students, 25 contact hours /year

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Ph.D. THESES SUPERVISED

EXAMINING COMMITTEES Ph.D. Theses

1979-1982 George Bobotas: Doctoral Thesis Committee, Graduate Department of Biochemistry, City University of New York

1979-1981Stanley Kirschenbaum: Doctoral Thesis Committee, Graduate Department of Biochemistry, City University of New York

1980-1983Thomas O’Hara: External Examiner, Doctoral Thesis Committee, Department of Anatomy, Wayne State University, School of Medicine

1978-1980Peter Simmons: External Examiner, Doctoral Thesis Committee, Department of Physiology, Yale University, School of Medicine

1986Akira Hishinuma: External Examiner, Doctoral Thesis Committee, Department of Biology, Columbia University

1986Diptendu Chattopadhya: External Examiner, Doctoral Thesis, Department of Biochemistry, University of Calcutta

1987-1992Maureen Talbot: Chair-Doctoral Thesis Committee, Graduate Department of Biochemistry, City University of New York

1992Christopher Turner: External Examiner, Doctoral Thesis Committee, Department of Neurosciences, University of Texas Medical Branch, Galveston

1993Yongquan Lo: External Examiner, Doctoral Thesis Committee, Department of Anatomy and Cell Biology, SUNY Health Science Center

1996Wei Zhang: External Examiner, Doctoral Thesis Committee, Graduate Department of Biochemistry, City University of New York

1996Michael Hayward: External Examiner, Doctoral Thesis Committee, Department of Biology, Columbia University

1996Heidi Clariss:- External examiner, Doctoral Thesis, University of Melbourne, Australia

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1997 1998 1996-2000 1998-2002

2003-2007 2003-2008 2004 2001-2005 2003-2005 2005

2005-2009 2005-2008 2007-2008 2006-2008 2006-08

2007-2011 2008-2009 2008-2012

2008-2012

2008

2009-2014 2009-2013 2009-2011 2009-2012 2009-2012 2010-2013 2011-2014 2013

2013 2014-present

Frank L. Margolis

Helen Treloar: External examiner, Doctoral Thesis, University of Melbourne, Australia

Simone Zehnter: External examiner, Doctoral Thesis, Griffith University, Queensland, Australia Gregory Carlson: Doctoral Thesis Committee, Department of Anatomy and Neurobiology, UMB Tian Shu-Doctoral Thesis Committee, Department of Anatomy and Neurobiology, UMB Jill Sharifi-Silverman: Doctoral Thesis Committee, UMB Program in Neuroscience

Renee Cockerham: Doctoral Thesis Committee, UMB Program in Neuroscience Aubrey Shoko: External examiner, Doctoral Thesis, University of Cape Town Yiling Nie: Doctoral Thesis Committee UMB Program in Neuroscience Ted Nelson: Doctoral Thesis Committee UMB Program in Neuroscience Kathleen McBride: Member, Doctoral Thesis examining committee, Dept of Psychology, American University

Mihyun Bae: Doctoral Thesis Committee UMB Program in Neuroscience Peter Murray: Doctoral Thesis Committee UMB Program in Neuroscience Cigdem Tosun: Doctoral Thesis Committee UMB Program in Neuroscience YiChun Hsieh: Doctoral Thesis Committee UMB Program in Neuroscience Nathan Wright: Doctoral Thesis Committee UMB Program in Biochemistry and Molecular Biology

Amanda Elson: Doctoral Thesis Committee UMB Program in Neuroscience Jennifer Laing: Doctoral Thesis Committee UMB Program in Microbiology Stephanie Parrish-Aungst: Doctoral Thesis Committee UMB Program in Neuroscience

Melissa Liriano: Doctoral Thesis Committee UMB Program in Biochemistry and Molecular Biology Jana Vukovic: External examiner, Doctoral Thesis 2008 University of Western Australia

Dushon Riley: Doctoral Thesis Committee UMB Program in Neuroscience Katelyn Niu: Doctoral Thesis Committee UMB Program in Neuroscience Sara Shelton Taylor: Doctoral Thesis Committee UMB Program in Neuroscience Akina Hoshino: Doctoral Thesis committee UMB Program in Neuroscience Bridget Nugent-: Doctoral Thesis Committee UMB Program in Neuroscience Cedric Uytingo: Doctoral Thesis Committee UMB Program in Neuroscience Adam Clark: Doctoral Thesis committee UMB Program in Toxicology Tom Fuller: Doctoral Thesis Committee Program in Molecular Medicine Tyler Demarest: Doctoral Thesis Committee UMB Program in Neuroscience SarahRudzinskas:DoctoralThesisCommitteeUMBPrograminNeuroscience

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Masters Theses

1978-1981Victoria Olejer: External Member, Masters Thesis Committee, Department of Biochemical Nutrition, Rutgers University

1981-1984Steven Greene: External Member, Masters Thesis Committee, Department of Biochemical Nutrition, Rutgers University (1981-1984).

1996Tanya Foster: Member, Masters Thesis Committee, Department of Anatomy and Neurobiology, UMB

1999Shen Du: Member, Masters Thesis Committee, Department of Anatomy and Neurobiology, UMB

2002Kathleen McBride-Member, Master’s Thesis examining committee, Dept of Psychology, American University

2013Mary Sedegagh: Masters Thesis Committee, Program in Molecular Medicine UMB

2013Aparna Baxi: Masters Thesis Committee, Program in Molecular Medicine UMB 2013Ankit Bhatta: Masters Thesis Committee, Program in Molecular Medicine UMB

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Frank L. Margolis 2013Laura Shoop: Masters Thesis Committee, Program in Molecular Medicine UMB

Mentor:

1989Thomas V. Getchell PhD: NRSA Senior Research Fellowship, “Molecular Neurobiology of Olfactory Reception,”

1993-1995Angela R. Porta PhD: NINDS Re-entry into Neurological Sciences Fellowship Award, “CASH: A Novel bHLH Protein: Role in Gene Transcription,”

2006-2007Mark Smiley BS: NIH-Minority Supplement 2006-2007

Pre-candidacy student advisory committees (Program in Neuroscience):

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2000 2001 2002 2002 2002 2004 2004-2006 2006-2007 2008-20009 2009-2010 2009-2011 2011-2013 2011-2013 2011-2012 2011-2013 2012-2013 2012-2013

Brian Polster Renee Cockerham Jimok Kim Alexis Hattox Erica Martin Jennifer Mackes Aubrey Siebert Stephanie Cerceo Bryan Chai Roger Mullins Katherine Friel Gilpin Nisha Pulimood Sarah Ransom Adam Clark-(Program in Toxicology) David Kurland Jonathan Van Ryzin Janell Payano Sosa

1996 1998-1999 2003-2004 2003 2004

Tanya Foster Tian Shu Hyun Hee Kim Ted Nelson Mei Hyun Bae

Student advisory committees (Dept. Anatomy/Neurobiology):

Lab. Rotation students-(UMB)

1997Zheng Xu 1997David Freilich 1998Brian Rash 1999Yuan Tian 1999Eric Lancaster 2001Yiling Nie 2002Gregory Bell 2003Hyun Hee Kim 2002Renee Cockerham 2004Carey Stamps 2004Shannon Campbell-MEDT 2008Sylvina Mullins

Positions and Employment

1966 – 1969  Asst. Res. Microbiologist, Dept. of Med. Microbiol. and Immunol., UCLA Sch. of Med.,

1969 – 1971  Research Assoc., Roche Institute of Molecular Biology

1971 – 1973   Asst. Member, Roche Institute of Molecular Biology .

1972 – 1995   Adjunct Professor, Graduate Program in Biochemistry, CUNY

1974 – 1980   Associate Member, Roche Institute of Molecular Biology .

1981 – 1995   Full Member, Roche Institute of Molecular Biology

1987 – 1988   Acting Chairman, Dept of Neurosci, Roche Institute of Molecular Biology

1988 – 1995   Head, Lab. Chemosensory Neurobiology, Roche Institute of Molecular Biology

1995 – date   Professor, Dept. of Anatomy and Neurobiology, University of Maryland, Baltimore.

2005 – date   Professor, Dept of Psychiatry, University of Maryland, Baltimore

2005 – date   Director, Neuroscience Graduate Program

Lab Techniques and Equipment

Lab Techniques and Equipment:

We utilize a range of biochemical, molecular and anatomical techniques in the lab such as 1) protein characterization by isolation, western blotting and cross linking, 2) cDNA cloning, RT-PCR, phage display and protein expression and 3) in situ hybridization, histology and immunocytochemistry. Behavioral and electrophysiological studies are by collaboration.

Selected Peer Reviewed Publications

Publications (selected from more than 200 total)

Other of importance to the field-

  • Pyrski, M, Koo, JH, Polumeri S, Ruknudin, A, Margolis,J, Schulze DH, Margolis, FL. Sodium-calcium exchanger expression in the mouse and rat olfactory systems. J. Comp. Neurol. 501:944-958 (2007). PMID: 17311327
  • Koo JH, Gill S, Pannell L, Menco B and Margolis FL The interaction of Bex and OMP reveals a metabolically active covalent dimer of OMP J. Neurochem. 90, 102-116 (2004). PMID: 15198671
    • Youngentob S. Pyrski, M. and Margolis FL, Adenoviral vector-mediated rescue of the OMP-null behavioral phenotype: Enhancement of odorant threshold sensitivity Behavioral Neuroscience 118: 636-642(2004). PMID: 15174942
    • Youngentob SL, P.F. Kent PF and Margolis FL OMP Gene Deletion Results in an Alteration in Odorant-Induced Mucosal Activity Patterns J. Neurophysiol. 90, 3864-3873.(2003) PMID: 12917392
    • Ivic, L, Pyrski, M, Margolis, J.W., Richards, L.J., Firestein S, and Margolis FL Adenoviral vector   mediated rescue of the OMP-null mouse.  Nature Neuroscience 3:1113-1120 (2000). PMID: 11036268
    • Youngentob SL, Margolis FL, Youngentob LM OMP gene deletion results in an alteration in odorant quality perception  Behav. Neurosci. 115: 626-631(2001). PMID: 11439451
    • Khazaei MR, Halfter H, Karimzadeh F, Koo JH, Margolis FL, Young P. J Bex1 is involved in the regeneration of axons after injury. Neurochem. 2010 Nov;115(4):910-20. doi: 10.1111/j.1471-4159.2010.06960.x. Epub 2010 Sep 28.