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Musab Samir Jafri, PhD

Academic Title:

Assistant Professor

Primary Appointment:

Neurology

Location:

Bressler Research Building, 11-045 (lab 11-056)

Phone (Primary):

(410) 706-2384

Education and Training

  • Assistant Professor: 2005-present: Department of Neurology,
  • Research Biologist: May, 2000-present, VA Medical Center, Baltimore, Baltimore, MD
  • Postdoctoral Fellow: 1997-2000, University of Miami School of Medicine, Miami FL - Stephen D. Roper, Ph.D.
  • Graduate Research Assistant (thesis research): 1993-1997, University of Maryland School of Medicine, Baltimore MD - Daniel Weinreich, Ph.D.
  • Graduate Research Assistant: 1992-1993, University of Maryland School of Medicine, Baltimore MD - Jay Yang, M.D., Ph.D.
  • Research Technician: 1986-1989, Duke University Eye Center, Durham NC - Alan Proia, M.D., Ph.D.
  • Undergraduate: BS, Chemistry with biological specialization, Duke University, Durham NC, 1985

Biosketch

My lab is primarily focused on neurodegenerative diseases, particularly Parkinson's disease, amyotrophic lateral sclerosis (ALS) and epilepsy.  Neurosurgical procedures are becoming an increasingly important therapeutic tool. The use of deep brain stimulating (DBS) electrodes for the treatment of Parkinson's disease is increasing and the use of DBS electrodes to treat disorders ranging from depression to obsessive-compulsive disorder to obesity to epilepsy is on the horizon.  One of the limitations for many of these treatments is the limited understanding of the connectivity of the brain, known as connectomics, in critical target regions for these therapies.  We have developed an ex vivo imaging method to create a detailed human brain map with resolution that's at least an order of magnitude better than MRI-derived maps.

We are developing a high-resolution three-dimensional atlas of the human basal ganglia using a technique developed in the lab called Anisotropic Scattering Imaging (ASI).  We have also constructed a novel illumination system that allows us to use optical anisotropy to identify individual tracts in the basal ganglia.  Using 100 um sections of human basal ganglia, we found that when light struck the sections from different angles, the images showed distinct white mater tracts within the internal capsule based. We concluded that we could use this information to deduce the trajectory of the fiber through the section and thus reconstruct distinct fiber tracts within the internal capsule – tracts that previously had not been resolved by standard techniques. This property called optical anisotropy can be seen in this video where the light is being projected onto the slide from different angles.

By reconstructing these fibers through sequential sections, we can elucidate a number of tracts shown in the images and videos below.

The pedunculopontine nucleus (PPN) has garnered considerable interest, because it's the only target by which deep brain stimulation (DBS) can substantially ameliorate the gait disturbances of Parkinson's disease. But no one has been able to visualize its connections in the human brain. With unprecedented resolution and precision, our method elucidates the distinct projections of the PPN. As can be seen in the image below and this video.

  • The green and red surfaced-rendered structures are the subthalamic (STN) and red nuclei, respectively.
  • The orange volume-rendered cloud represents dopaminergic cells of the substantianigra (SN).
  • The blue volume-rendered cloud represents choline-acetyltransferase-positive cells of the PPN.
  • Tracts with poorly saturated colors represented poorly myelinated projections.
  • The pale blue tracts represent the pedunculosubthalamic fibers, connecting the PPN and the STN.
  • The pale yellow tracts represent the pedunculothalamic fibers, connecting the PPN and thalamus.
  • The pale orange tracts represent the connections between the PPN and the SN. The pale green tracts represent the connections between the STN and the SN.
  • The gray and black surfaced rendered structures are the anterior and posterior commissures, respectively, rendered for the purpose of anatomical orientation.

Other tract segments are arbitrarily highlighted with colors of full saturation, to indicate myelination. The internal capsule is purple, the subthalamic fasciculus red, the lenticular fasciculus cyan, and thalamic tracts orange. [click blue txt]

Research/Clinical Keywords

Neurodegeneration, neuroprotection, Parkinson's disease, amyotrophic lateral sclerosis, ALS, Lou Gehrig's disease, brain mapping, connectomics, optical coherence tomography, OCT, imaging,

Highlighted Publications

Shin RK, Qureshi RA, Harris NR, Bakar D, Li TP, Jafri MS, and Tang C-M.  Wilbrand’s knee, Neurology, 82(5):459-60, 2014.

Liang C-P, Wu Y, Schmitt J, Bigeleisen PE, Slavin J, Jafri MS, Tang C-M, Chen Y, Coherence-gated Doppler: a fiber sensor for precise localization of blood flow. Biomedical Optics Express, 4(5), 760-71, 2013.

Liang C-P, Wierwille J, Moreira T, Schwartzbauer G, Jafri MS, Tang C-M, Chen Y,  A forward-imaging needle-type OCT probe for image guided stereotactic procedures. Optics Express, 19(27), 26283-94, 2011.

McDowell KA, Shen W-B, Siebert AS, Clark SM, Dugger NV, Valentino KM, Wilson JM, Jinnah HA, Fishman PS, Shaw CA, Jafri MS, Yarowsky PJ, Environmental neurotoxin-induced progressive model of parkinsonism in rats, Annals of Neurology, 68(1), 70-80, 2010.

Siebert AS, Desai V, Chandrasekaran K, Fiskum G, Jafri MS, Nrf2 activators provide neuroprotection against 6-hydroxydopamine toxicity in rat organotypic nigrostriatal co-cultures, Journal of Neuroscience Research, 87(7), 1659-69, 2009.

Jafri MS, Tang R, Tang C-M, Optical coherence tomography guided neurosurgical procedures in small rodents, Journal of Neuroscience Methods, 176(2), 85-95, 2009.

Jafri MS, Schmitt JM, Farhang S, Tang RS, Desai N, Fishman PS, Rohwer RG, Tang C-M, Optical coherence tomography in the diagnosis and treatment of neurological disorders, Journal of Biomedical Optics, 10(5), 051603(1-11), 2005.

Additional Publication Citations

Dissertation

Jafri MS, Autacoid Modulation of Vagal Primary Afferent Neuron Excitability, Ph.D. Dissertation, Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Advisor: Dr. Daniel Weinreich, 1997

Publications

Shin RK, Qureshi RA, Harris NR, Bakar D, Li TP, Jafri MS, and Tang C-M.  Wilbrand’s knee, Neurology, 82(5):459-60, 2014.

Liang C-P, Wu Y, Schmitt J, Bigeleisen PE, Slavin J, Jafri MS, Tang C-M, Chen Y, Coherence-gated Doppler: a fiber sensor for precise localization of blood flow. Biomedical Optics Express, 4(5), 760-71, 2013.

Liang C-P, Wierwille J, Moreira T, Schwartzbauer G, Jafri MS, Tang C-M, Chen Y,  A forward-imaging needle-type OCT probe for image guided stereotactic procedures. Optics Express, 19(27), 26283-94, 2011.

McDowell KA, Shen W-B, Siebert AS, Clark SM, Dugger NV, Valentino KM, Wilson JM, Jinnah HA, Fishman PS, Shaw CA, Jafri MS, Yarowsky PJ, Environmental neurotoxin-induced progressive model of parkinsonism in rats, Annals of Neurology, 68(1), 70-80, 2010.

Published responses to letters to the editor for the above paper:

McDowell KA, Shen W-B, Siebert AS, Clark SM, Jinnah HA, Sztalryd C, Fishman PS, Shaw CA, Jafri MS, Yarowsky PJ, Washed cycad flour contains b-N-methylamino-LAlanine and may explain Parkinsonian symptoms, Annals of Neurology, 69(2), 423-4, 2011.

McDowell KA, Shen W-B, Siebert AS, Clark SM, Jinnah HA, Sztalryd C, Fishman PS, Shaw CA, Jafri MS, Yarowsky PJ, Neurotoxic cycad components and Western Pacific ALS/PDC, Annals of Neurology, 68(6), 976, 2010.

Siebert AS, Desai V, Chandrasekaran K, Fiskum G, Jafri MS, Nrf2 activators provide neuroprotection against 6-hydroxydopamine toxicity in rat organotypic nigrostriatal co-cultures, Journal of Neuroscience Research, 87(7), 1659-69, 2009.

Jafri MS, Tang R, Tang C-M, Optical coherence tomography guided neurosurgical procedures in small rodents, Journal of Neuroscience Methods, 176(2), 85-95, 2009.

Lin J, Staecker H, Jafri MS.  OCT imaging of the inner ear: A feasibility study with implications for cochlear implantation, Annals of Otology, Rhinology & Laryngology, 117, 341-6, 2008.

Laing JM, Golembewski EK, Wales SQ, Liu J, Jafri MS, Yarowsky PJ, Aurelian L. The growth compromised HSV-2 vector ΔRR protects from NMDA-induced neuronal degeneration through redundant activation of the MEK/ERK and PI3-K/Akt survival pathways either one of which overrides apoptotic cascades, Journal of Neuroscience Research, 86(2), 378-91, 2008.

Burris N, Schwartz K, Tang C-M., Jafri MS, Schmitt J, Kwon MH, Toshinaga O, Gu J, Brown J, Brown E, Pierson R, Poston R. Catheter-Based Infrared Light Scanner as a Tool to Assess Conduit Quality in Coronary Artery Bypass Surgery, Journal of Thoracic and Cardiovascular Surgery, 133(2), 419-27, 2007.

Jafri MS, Schmitt JM, Farhang S, Tang RS, Desai N, Fishman PS, Rohwer RG, Tang C-M, Optical coherence tomography in the diagnosis and treatment of neurological disorders, Journal of Biomedical Optics, 10(5), 051603(1-11), 2005.

Caicedo A, Jafri MS, Roper SD, In situ imaging reveals neurotransmitter receptors for glutamate in taste receptor cells, Journal of Neuroscience, 20(21), 7978-7985, 2000.

Jafri MS and Weinreich D, Substance P regulates IK and Ih decreases excitability of ferret vagal sensory neurons via a NK-1 receptor, Journal of Neurophysiology, 79, 769-777, 1998.

Jafri MS, Moore KA, Taylor GE, Weinreich D, Histamine H1 receptor activation blocks two classes of potassium current, IK(leak) and IAHP, to excite ferret vagal afferents, Journal of Physiology (London),503(3), 533-546, 1997.

Cohen AS, Moore KA, Bangalore R, Jafri MS, Weinreich D, Kao JPY, Ca2+-induced Ca2+ release mediates Ca2+ transients evoked by single action potentials in rabbit vagal afferent neurones, Journal of Physiology (London), 499(2), 315-28, 1997.

Jafri MS and Weinreich D, Substance P hyperpolarizes vagal sensory neurones of the ferret, Journal of Physiology (London), 493(1), 157-66, 1996.  [Published erratum, Journal of Physiology (London), 494(3)]

Isenberg KE, Ukhun IA, Holstad SG, Jafri S, Uchida I, Zorumski CF, Yang J, Partial cDNA cloning and NGF regulation of a rat 5-HT3 receptor subunit, Neuroreport, 5(2), 121-4, Nov 1993.

Wiggins RE, Jafri MS, Proia AD, 12(S)-hydroxy-5,8,10,14-eicosatetraenoic acid is a more potent neutrophil chemoattractant than the 12(R) epimer in the rat cornea, Prostaglandins, 40(2), 131-41, Aug 1990.

Hall IH, Spielvogel BF, Sood A, Ahmed F, Jafri S, Hypolipidemic activity of trimethylamine-carbomethoxyborane and related derivatives in rodents, J Pharm Sc, 76(5), 359-65, 1987.

Abstracts

Moreira TH, Li TP, Jafri MS, Krause GB, Tang C-M, A high resolution, 3D atlas of the fiber tracts near the human STN based on optical anisotropy, Society for Neuroscience Annual Meeting, 2011

Siebert AA, Yarowsky PJ, Jafri MS; Nrf2 activation is neuroprotective using a post-lesion treatment strategy in a progressive organotypic model of Parkinson’s disease, Society for Neuroscience Annual Meeting, 2011

Jafri MS, Comeau R, Frey S, Tang R, Tang C-M, A Delivery System for Stem Cells, The 1st Maryland Stem Cell Research Symposium, December 2008

Siebert A and Jafri MS. Nrf2 activation is neuroprotective using a post-lesion treatment strategy in a progressive organotypic model, Society for Neuroscience Annual Meeting, 2008

Siebert A, Desai V, Chandrasekaran K, Siebert A, Fiskum G, Jafri MS, Nrf2 protects dopaminergic neurons against 6-OHDA toxicity in nigrostriatal organotypic cocultures, Society for Neuroscience Annual Meeting, 2006

Desai V, Chandrasekaran K, Siebert A, Fiskum G, Jafri MS, Nrf2 activation protects DA neurons from 6-OHDA in nigrostriatal organotypic co-cultures, Baltimore Chapter of the Society for Neuroscience, 2006

Tang C-M and Jafri MS, Steerable trajectory with optical guidance: A more efficient means for DBS mapping, Neural Interfaces 2005, NINDS, NIH, 2005

Desai V, Chandrasekaran K, and Jafri MS, Oxidative stress-induced gene expression via nrf2 in Parkinson’s disease, UMB/NIH High School Biomedical Research Program, 2005

Jafri MS, Kahraman S, Tang C-M, Fiskum G, Mitochondrial imaging in nigrostriatal organotypic coculture, Mitochondria and Neuroprotection Symposium, 2004

Do K, Jafri MS, Staecker H, Optical coherence tomography: Temporal bone applications, American Academy of Otolaryngology Meeting, 2004

Tang C-M, Schmitt JM, Jafri MS, OCT applications in the brain and spinal cord, Optical Imaging 2004: Optical Diagnostic Imaging from Bench to Bedside at the National Institutes of Health, 2004

Tuo L, Desai N, Jafri MS, Developing a novel model for Parkinson's disease, UMB/NIH High School Biomedical Research Program, 2004

Jafri MS, Carter M, Fishman P, Tang C-M, Optical coherence tomography (OCT) of human basal ganglia, Society for Neuroscience Annual Meeting, 2003

Desai N and Jafri MS, Assessment of dopaminergic neurodegeneration using organotypic nigrostriatal co-culture, UMB/NIH High School Biomedical Research Program, 2002

Lanio E, Tang C-M, Jafri MS, Developing an in vitro model for Parkinson’s disease, NIH High School Biomedical Summer Research Program, 2001

Jafri MS and Roper SD, Real-time measurement of neurotransmitter release from rat taste buds, Association for Chemoreception Sciences Annual Meeting, 2000.

Caicedo A, Johnson DM, Jafri MS, Roper SD, Calcium imaging reveals synaptic glutamate receptors in taste cells, Association for Chemoreception Sciences Annual Meeting, 2000.

Jafri MS and Roper SD, Identification of neurotransmitter release at rat taste bud synapses, Society for Neuroscience Annual Meeting, 1999.

Jafri MS and Roper SD, Taste stimuli induce transmitter release at rat taste bud synapses in vitro, Association for Chemoreception Sciences Annual Meeting, 1999.

Jafri MS, Kamatchi G, Kao JPY, Weinreich D, Nicotinic receptors mediate a calcium-dependent decrease in membrane excitability in vagal afferents in vitro, Society for Neuroscience Annual Meeting, 1997.

Jafri MS and Weinreich D, Substance P regulation of IK and Ih decreases excitability of ferret vagal sensory neurons via a NK-1 receptor, Society for Neuroscience Annual Meeting, 1996.

Moore KA, Jafri MS, Weinreich D, Reduction of resting potassium conductance contributes to histamine depolarization of vagal sensory neurons. FASEB Journal,10, A163, 1996.

Jafri MS and Weinreich D, Characterization of a novel hyperpolarizing response to substance P in ferret nodose neurons, Society for Neuroscience Annual Meeting, 1994.

Ukhun IA, Holstad SG, Jafri S, Uchida I, Yang J, Isenberg KE, NGF regulation 5-HT3 receptor expression, Society for Neuroscience Annual Meeting, 1993.

Wiggins RE, Jafri MS, Proia AD, Comparison of neutrophil influx following injection of 12(S)- and 12(R)-HETE into the stroma of rat corneas, Investigative Ophthalmology & Visual Sciences, 29 (Suppl.), 288, 1988.

Proia AD, Jafri MS, Klintworth GK, Lipid crystals in orbital phycomycosis, Investigative Ophthalmology & Visual Sciences, 28(3) (Suppl.), 31, 1987.