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Molecular Genetics & Nutrigenomics (MGN) Core

Core Requests

Submit a Core Request. 

Director: Braxton Mitchell, M.P.H., Ph.D.

Co-Director:  Alan Shuldiner, M.D.

Contact: bmitchel@medicine.umaryland.edu for further information.

The MGN Core will support a major goal of our center, to discover genes and gene-nutrient interactions associated with risk of obesity, diabetes, hypertension, atherogenesis and longevity and understand their molecular, cellular and physiological roles in the etiology of obesity, diabetes, and associated metabolic abnormalities.  Thus, this Core will provide investigators with access to large genotyped population databases, assist investigators with access to gene chip technology, unique Expressed Sequence Tags (EST) libraries, and bioinformatic tools.  Importantly, the Core will facilitate studies of the genetic underpinnings in phenotypic responses to nutrition interventions such as weight loss or specific dietary components.

The objectives of the MGN Core are to provide the following services and support for NORC investigators:

Molecular Genetics

  • DNA sequence analysis
  • High throughput short tandem repeat (STR) analysis
  • High throughput single nucleotide polymorphism (SNP) analysis
  • Mutation detection by denaturing high performance liquid chromatography (dHPLC)
  • DNA extraction and quantitation
  • Other general molecular biology and genetics methods (PCR, primer design, site directed mutagenesis, cDNA and genomic cloning)

Genetic Epidemiology

  • Data entry and management
  • Study design and power calculations
  • Association and linkage analysis
  • Haplotype construction and analysis
  • Access to Banked DNA, Serum and Plasma

Functional Genomics

  • cDNA microarray analysis (probe labeling, hybridization, washing, scanning)
  • Bioinformatics and microarray data analysis
  • Real time RT-PCR