Adipose Biology & Basic Mechanisms (ABBM) Core
Director: Carole Sztalryd, Ph.D.
Co-Directors: John McLenithan, Ph.D. and Da-Wei Gong, M.D., Ph.D.
The ABBM Core will facilitate access of investigators to adipose tissue samples from well-characterized subjects, and assist investigators with analysis of adipose tissue morphology and metabolism, specialized cell and organ culture methods, and analysis of gene and protein expression. This Core will enable investigators to accurately phenotype adipose tissue and adipocyte functions and to assess the functional importance of their gene or protein of interest.
The Core will bank tissue and RNA samples from well-characterized human subjects undergoing weight loss and nutrition interventions, as well as tissue and cell samples from relevant cell and organ culture studies. The Core will also provide consultation on the study of adipose tissue in animal models of obesity, diabetes, and caloric restriction to allow members to quickly and efficient test disease regulation of their gene or protein or interest.
An important function of this Core is to facilitate mechanistic cell and molecular studies in tissue samples from clinical researches who mainly study whole body metabolism, and vice versa, to allow basic researcher understand the relevance of their findings in cell and animal models to human physiology and pathophysiology.
ABBM Core Objectives
- Provide investigators with standardized acquisition of adipose tissue samples and assessment of adipose morphology, metabolism, gene and protein expression performed by expert technical staff.
- Provide easy access to well-characterized cell and organ culture systems, and specialized technologies for adipose and other cell types (as needed).
- Provide training to students, fellows, and investigators in specialized methods of adipose biology, including microscopy and gene transfer technologies.
- Develop new technologies as needed by a critical mass of NORC investigators.