Skip to main content
  • Scientific Core Facilities Fueling Cancer Center’s Success

    Kevin Cullen, M.D., Director, Marlene and Stewart Greenebaum Comprehensive Cancer Center discusses the important role the Center for Innovative Biomedical Resources (CIBR) have played in the Cancer Center’s success.

    Watch the Video

Confocal Microscopy Facility

The Confocal Core’s mission is to provide researchers with a wide array of state-of-the-art confocal imaging equipment to enable acquisition of high resolution images (both in vivo and in vitro). The Confocal Core offers training and assistance in the use of multiple confocal microscopes housed in our facility. Optimization of data acquisition and image processing are both part of the training, thus enabling researchers to efficiently design studies, acquire image data and extract relevant data features.

The confocal facility is available to all UMB researchers and extramural users on a fee-for-service basis.

Scheduler Links

Zeiss 710 NLO & Zeiss 7MP

  • Upright confocal microscope with single photon and multiphoton excitation capabilities for imaging live cells, slices and whole animals
  • Excitation wavelengths 730 to 1300 nm; 2 PMT and 2 sensitive GaAsP detectors
  • Can be combined with electrophysiology or other measures

Zeiss 5Live & Zeiss 510

  • Point-scanning and slit-scanning confocal microscope
  • Fast acquisition frame rates for studying dynamic cellular processes at physiological temperatures
  • Dual scan heads (5Live) allow simultaneous imaging and optical manipulation
  • Excitation (488, 543, 560, 633, Ti:Sapphire laser)

The facility provides individual instruction on an array of confocal microscopes. The needs of the researcher are considered in choosing which microscope will best suit the experimental design. In general, imaging of fixed samples, cultured cells, organ slices and small animals can be accommodated. Imaging techniques including FRET, FRAP, photoactivation and uncaging are readily implemented.

The microscopes have excitation sources that cover most fluorophores with excitation ranging from 355- 633nm. Multiphoton excitation of fluorophores is also available on select instruments. An image analysis workstation equipped with software packages is available to users.

The core also has a culture room with an incubator, culture hood and a widefield fluorescence microscope for use in preparation of cultured and live samples. Preparation of live animals for imaging experiments can also be done in this space.


Blanpied_ThomasThomas Blanpied, PhD
Program Director

tblanpied@som.umaryland.edu
410-706-4769
660 West Redwood Street, HH 505
Baltimore, MD 21201

 

MAUBAUN_JOSEPHJoseph Ryan H. Mauban, PhD
Confocal Core Manager

jmauban@som.umaryland.edu
410-706-6170
660 West Redwood treet, HH 523
Baltimore, MD 21201

 


Services We Offer

  • Training
  • LSM DUO
  • LSM510 META
  • Automic Force Microscopy
  • Workstation

Get pricing & info