Confocal Microscopy Facility
The confocal facility is available to all UMB researchers and extramural users on a fee-for-service basis.
- Upright confocal microscope with single photon and multiphoton excitation capabilities for imaging live cells, slices and whole animals
- Excitation wavelengths 730 to 1300 nm; 2 PMT and 2 sensitive GaAsP detectors
- Can be combined with electrophysiology or other measures
- Point-scanning and slit-scanning confocal microscope
- Fast acquisition frame rates for studying dynamic cellular processes at physiological temperatures
- Dual scan heads (5Live) allow simultaneous imaging and optical manipulation
- Excitation (488, 543, 560, 633, Ti:Sapphire laser)
The facility provides individual instruction on an array of confocal microscopes. The needs of the researcher are considered in choosing which microscope will best suit the experimental design. In general, imaging of fixed samples, cultured cells, organ slices and small animals can be accommodated. Imaging techniques including FRET, FRAP, photoactivation and uncaging are readily implemented.
The microscopes have excitation sources that cover most fluorophores with excitation ranging from 355- 633nm. Multiphoton excitation of fluorophores is also available on select instruments. An image analysis workstation equipped with software packages is available to users.
The core also has a culture room with an incubator, culture hood and a widefield fluorescence microscope for use in preparation of cultured and live samples. Preparation of live animals for imaging experiments can also be done in this space.