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Biosensor Core

Our scientific objective is to provide the faculty, staff and students on our campus with the latest technology for the quantitative study of binding reactions in real time, specifically with an approach that is versatile, highly sensitive, and “user
friendly”, with molecules that are label-free.

The instruments we use for this purpose are from Biacore® (GE Healthcare) – the T200 and 3000.

Biacore® instrumentation is available for your use in the Biosensor Core Facility maintained by the Department of Physiology, University of Maryland School of Medicine.

Biacore® sensor technology provides a versatile, highly sensitive, easy-to-use, and quantitative approach to study the kinetics and affinity of binding interactions under exquisitely controlled conditions.

Visit the Biosensor Core website to learn more.


Yinghua Zhang

401-706-2036

YZhan004@umaryland.edu

Robert J. Bloch Ph.D.Robert Bloch

410-706-3020

RBloch@umaryland.edu


Services We Offer

  • Biacore® instruments utilize the optical method of “surface plasmon resonance“ (SPR), small changes in the interaction of monochromatic light with a metallic surface that occur when a protein or other molecule binds to that surface.
  • Using the T200 or 3000, the core and its staff can provide accurate determinations of “on” and “off” rates for binding reactions, as well as determine affinity constants for binding.
  • As it uses SPR, many different kinds of binding reactions can be studied, often robotically, and a wide range of biological molecules can be examined, including proteins, nucleic acids, carbohydrates and lipids, as well as small molecules. Typical studies can:
    • Determine if pairs of molecules bind to each other.
    • Determine kinetic constants, binding constants, and specificity of binding.
    • Determine if several molecules can bind simultaneously to the same ligand or if they compete for binding. 

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