Skip to main content
  • Cytometry Shared Service Banner01

Flow Cytometry Shared Service

MISSION:

The University of Maryland Greenebaum Comprehensive Cancer Center Flow Cytometry Shared Service (FCSS) offers equipment and technical expertise to the entire campus, as well as outside clients in conducting research in all areas of basic and applied biomedical sciences.


SERVICES:

  • Experimental design, troubleshooting, consultation and grant application
  • Cell sorting performed by staff
  • Monthly lecture covers basic knowledge, useful tools, and new development. Free and open to all.
  • Hands on training on analyzers (LSR II, Canto II, Accuri C6, and FlowSight) Investigators are encouraged to use the analyzers independently.
  • Training on analysis software

INSTRUMENATION:

Cytek Aurora Spectral Cytometer

  • 3 lasers: 488 nm, 405 nm, 640 nm
  • > 20 color detection
  • Use 38 high sensitive APD detectors to read the whole spectrum of each color
  • Resolve colors with similar emission spectrums
  • Small particle detection: 100 nm range

Cytek Aurora Spectral Cytometer

Red represents the spillover from the dye in the row to the dye in column

Cytometry-Shared01Amnis FlowSight Imaging Cytometer

  • 4 lasers: 407, 488, 552, 640 nm, 405nm, 561nm
  • 10 colors
  • 20X magnification
  • Autosampler

Cytometry-Shared02

Cytometry-Shared03BD Aria II Cell Sorter

  • 4 lasers: 488 nm, 633 nm, 405 nm, 552 nm
  • 11 colors
  • 96-well single cell sorting, 4-way sorting
  • Index sorting
  • BSL-2 enhanced sorting inside of bioBUBBLE enclosure

Cytometry-Shared04BD LSR II

  • 4 lasers: 488 nm, 640 nm, 405 nm, 561 nm
  • 13 colors
  • High throughput sampler

Cytometry-Shared05BD CANTO II

  • 2 lasers: 488 nm, 640 nm
  • 6 colors

Cytometry-Shared06

BD Accuri C6

  • 2 lasers: 488 nm, 640 nm
  • 4 colors
  • Volume measurement
  • Located in HSF II, S107

ANALYSIS SOFTWARE

Cytometry-Shared07FCS Express 6 Site License

No charge for facility users

 

 

Cytometry-Shared08FlowJo Dongles

PC and Mac dongles can be borrowed at no charge.

Order Online


CONTACT:

Fan_Xiaoxuan

Xiaoxuan Fan, PhD

Director

UNDERWOOD_KAREN

Karen F. Underwood, B.S.

Research Lead Specialist

Bryan Hahn, BS

Cell Sorter Operator


LOCATION:

Room 7-022, Bressler Research Building
685 West Baltimore Street
Baltimore, MD 21201


HOURS:

Monday through Friday
7:00am - 5:00pm


PHONE:

Office: 410-706-2173
Fax: 410-328-6559

Email:


LABORATORY POLICIES:

Experiments should preferably be scheduled one to two weeks in advance.

All sample analysis and cell sorting is done by Core Laboratory personnel.

The “Rules and Regulations” form (Revision March 10, 2015) is available at the CVD Flow Cytometry Core Laboratory

Download Brochure